Research Article
Characterization and Development of EST-SSRs by Deep Transcriptome Sequencing in Chinese Cabbage (Brassica rapa L. ssp. pekinensis)
Table 3
Details of 19 EST-SSRs that successfully yielded PCR amplicons in FuShanBaoTou.
| | Code | EST-SSR name | Motif | Product size expected (bp) | Product size validated (bp) | SSR location | Number of alleles |
| | BR-es1 | CL3455.Contig1_All-2 | (TC)11 | 97 | 97 | CDS | 2 | | BR-es2 | CL4114.Contig2_All-2 | (TCA)7 | 157 | 157 | 3-UTR | 3 | | BR-es3 | CL2525.Contig4_All-1 | (TAG)9 | 160 | 160 | CDS | 4 | | BR-es4 | Unigene10387_All-1 | (CTC)9 | 127 | 127 | CDS | 2 | | BR-es5 | CL7077.Contig2_All-1 | (AATC)5 | 153 | 153 | 3-UTR | 3 | | BR-es6 | Unigene16359_All-1 | (AACC)5 | 134 | 130 | 5-UTR | 1 | | BR-es7 | CL4685.Contig1_All-1 | (CCTT)6 | 160 | 148 | 3-UTR | 2 | | BR-es8 | CL5247.Contig3_All-1 | (TTTC)6 | 133 | 141 | CDS | 3 | | BR-es9 | CL3462.Contig4_All-1 | (AATCG)4 | 155 | 155 | CDS | 2 | | BR-es10 | CL5726.Contig2_All-2 | (TCTCT)4 | 146 | 146 | 5-UTR | 3 | | BR-es11 | Unigene6713_All-1 | (AAAAC)4 | 118 | 118 | CDS | 1 | | BR-es12 | CL7282.Contig2_All-1 | (GAGGA)5 | 140 | 117 | CDS | 4 | | BR-es13 | Unigene2970_All-1 | (GAACT)5 | 106 | 106 | 3-UTR | 3 | | BR-es14 | Unigene8739_All-1 | (GATTT)5 | 130 | 130 | 5-UTR | 4 | | BR-es15 | CL5873.Contig4_All-2 | (CCCTAA)4 | 146 | 146 | 3-UTR | 4 | | BR-es16 | Unigene14449_All-1 | (CTCAAG)5 | 99 | 185 | CDS | 6 | | BR-es17 | Unigene5096_All-1 | (ACTCCC)5 | 141 | 141 | CDS | 3 | | BR-es18 | CL4691.Contig2_All-1 | (GATGGT)7 | 155 | 117 | CDS | 6 | | BR-es19 | Unigene13507_All-1 | (ATTTG)4 | 152 | 152 | CDS | 2 |
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EST-SSRs shown in bold have sizes different from the expected sizes.
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