International Journal of Genomics / 2015 / Article / Tab 1 / Research Article
Heat Shock Protein 70 and 90 Genes in the Harmful Dinoflagellate Cochlodinium polykrikoides : Genomic Structures and Transcriptional Responses to Environmental Stresses Table 1 The primers used in the present study.
Gene Primer Remark Nucleotide sequence ( CpHSP90 CpH90F1 RT-PCR GTTCGACACCTCACTGCTCACC CpHSP90 CpH90R1 RT-PCR AGGCCGAGCTTGATCATGC CpHSP70 CpH70F1 RT-PCR GGGCAGACCTTCACGACGTATG CpHSP70 CpH70R1 RT-PCR AATGCCGTCCAAGTGGAACTTC Cp18S Cp18S-F RT-PCR GGAGTATGGTCGCAAGGCTGAAAC Cp18S Cp18S-R RT-PCR CCTCGTGTTGAGTCAAATTAAGCC CpHSP90 CpHSP90-3F2 3′-RACE TGCTCACCTCCGGCTTCAAC CpHSP90 CpHSP90-3F3 3′-RACE CCACCGCATGATCAAGCTC CpHSP90 CpHSP90-5R2 5′-RACE ATGCGGCTGCTGATGATG CpHSP90 CpHSP90-5R3 5′-RACE ACCTTCTCCACCTTGTCGC CpHSP90 CpHSP90-SR1 Full length GTGTCCAAGTCTTGTGATCGAC CpHSP90 CpHSP90-SR2 Full length AGCACTAGCGCCGGTCATGT CpHSP90 5-SL 5′-RACE/full length CGTAGCCATTTTGGCTCAAG CpHSP70 CpHSP70-3F1 3′-RACE CGCTGCAGGAGGAGAAGTTG CpHSP70 CpHSP70-3F2 3′-RACE TGGAGGGCGTTGTCAATC CpHSP70 CpHSP70-SF1 Full length TTTCTCGGGGGTTTTGCTTCG CpHSP70 CpHSP70-SF2 Full length CATGGCGAAAAAGACGGCTGTT CpHSP70 CpHSP70-SR1 Full length TGGGACAGTCTGGGGTTGCT CpHSP70 CpHSP70-SR2 Full length ACACTCGGCGAATCCTCAG B26 3′-RACE GACTCTAGACGACATCGA(T)18 B25 3′-RACE GACTCTAGACGACATCGA CpHSP70 CpHSP70-DF1 Genomic DNA TGTCAGTCATCCCCTATTTGTC CpHSP70 CpHSP70-DR1 Genomic DNA GACAGTCTGGGGTTGCTGAATT CpHSP90 CpHSP90-DF1 Genomic DNA TGGGAAACCATTTTTGAGCATTG CpHSP90 CpHSP90-DR1 Genomic DNA TGCAGGTGTGAACCACTCAGC CpHSP70 CpHSP70-IF1 Intergenic DNA GAGGGCGTTGTCAATCCCATG CpHSP70 CpHSP70-IR1 Intergenic DNA AATGCCGTCCAAGTGGAACTTC
