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International Journal of Genomics
Volume 2015, Article ID 528395, 12 pages
Research Article

Transcriptomes That Confer to Plant Defense against Powdery Mildew Disease in Lagerstroemia indica

1USDA-ARS, Crop Germplasm Research, College Station, TX 77845, USA
2Texas A&M AgriLife Research and Extension Center, Dallas, TX 75252, USA
3Department of Plant Pathology, Texas A&M University, College Station, TX 77843, USA
4USDA-ARS, Thad Cochran Southern Horticultural Laboratory, Poplarville, MS 39470, USA

Received 30 November 2014; Accepted 6 May 2015

Academic Editor: Giuliana Napolitano

Copyright © 2015 Xinwang Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Transcriptome analysis was conducted in two popular Lagerstroemia cultivars: “Natchez” (NAT), a white flower and powdery mildew resistant interspecific hybrid and “Carolina Beauty” (CAB), a red flower and powdery mildew susceptible L. indica cultivar. RNA-seq reads were generated from Erysiphe australiana infected leaves and de novo assembled. A total of 37,035 unigenes from 224,443 assembled contigs in both genotypes were identified. Approximately 85% of these unigenes have known function. Of them, 475 KEGG genes were found significantly different between the two genotypes. Five of the top ten differentially expressed genes (DEGs) involved in the biosynthesis of secondary metabolites (plant defense) and four in flavonoid biosynthesis pathway (antioxidant activities or flower coloration). Furthermore, 5 of the 12 assembled unigenes in benzoxazinoid biosynthesis and 7 of 11 in flavonoid biosynthesis showed higher transcript abundance in NAT. The relative abundance of transcripts for 16 candidate DEGs (9 from CAB and 7 from NAT) detected by qRT-PCR showed general agreement with the abundances of the assembled transcripts in NAT. This study provided the first transcriptome analyses in L. indica. The differential transcript abundance between two genotypes indicates that it is possible to identify candidate genes that are associated with the plant defenses or flower coloration.