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International Journal of Genomics
Volume 2015 (2015), Article ID 679548, 7 pages
http://dx.doi.org/10.1155/2015/679548
Research Article

Expressed Sequence Tags Analysis and Design of Simple Sequence Repeats Markers from a Full-Length cDNA Library in Perilla frutescens (L.)

1Bioherb Research Institute, Kangwon National University, Chuncheon 200-701, Republic of Korea
2Department of Bioconvergence Science and Technology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea
3Hwajin Cosmetics, Hongcheon 250-807, Republic of Korea
4Department of Agricultural Life Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea
5Department of Applied Bioscience, Konkuk University, Seoul 143-701, Republic of Korea

Received 27 July 2015; Accepted 21 October 2015

Academic Editor: Xiaohan Yang

Copyright © 2015 Eun Soo Seong et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Perilla frutescens is valuable as a medicinal plant as well as a natural medicine and functional food. However, comparative genomics analyses of P. frutescens are limited due to a lack of gene annotations and characterization. A full-length cDNA library from P. frutescens leaves was constructed to identify functional gene clusters and probable EST-SSR markers via analysis of 1,056 expressed sequence tags. Unigene assembly was performed using basic local alignment search tool (BLAST) homology searches and annotated Gene Ontology (GO). A total of 18 simple sequence repeats (SSRs) were designed as primer pairs. This study is the first to report comparative genomics and EST-SSR markers from P. frutescens will help gene discovery and provide an important source for functional genomics and molecular genetic research in this interesting medicinal plant.