Expressed Sequence Tags Analysis and Design of Simple Sequence Repeats Markers from a Full-Length cDNA Library in Perilla frutescens (L.)

Table 3

EST-SSR primer pairs produced in EST (expressed sequence tags) sequencing database of Perilla frutescens.

Number	Unigene ID	Repeat motifs	Left primer sequence	Tm.	Right primer sequence	Tm.	Product size (bp)	Annotation (NR DB)
1	Contig 17	ATCAT()	GAGAGTATAAACAAATCCAAAACAGC	58.795	AGCCGGTATATCCAATTCCC	60.006	562	PREDICTED: protein CURVATURE THYLAKOID 1B, chloroplastic [Sesamum indicum]
2	Contig 67	A()	AGCAACTGCGGGTAGCTAGA	60.176	CAATCCGACCACAGTTGATG	59.96	172	PREDICTED: photosystem I subunit O [Sesamum indicum]
3	Perilla-1-1a_pTriplEx2-seq_C16	GA()	AGCGTACTGTTGAAAGCGTG	59.148	CAGCAAACGTGCTCGAATTA	60.014	247	PREDICTED: uncharacterized protein LOC105172991 isoform X2 [Sesamum indicum]
4	Perilla-1-1a_pTriplEx2-seq_E18	CTT()	GCCAATTTGAAGCTTTAGCC	58.969	GAATGTGAAGTGGGAACGCT	60.119	773	PREDICTED: GRF1-interacting factor 3 [Sesamum indicum]
5	Perilla-1-1a_pTriplEx2-seq_M02	AGAATG()	TGGAGCAAGTGAAGCAACAG	60.175	CCTTTTCAGTGAGGAGCCAG	59.982	191
6	Perilla-1-2a_pTriplEx2-seq_A02	TTTTG()	AATGATGGGTGTGATGAGCA	59.925	AAAGAATTTGAAGGCGCAGA	59.96	401	PREDICTED: homeobox-leucine zipper protein HAT5-like [Sesamum indicum]
7	Perilla-1-3a_pTriplEx2-seq_B13	ATCAT()	GAGAGTATAAACAAATCCAAAACAGC	58.795	CGGTATATCCAATTCCCACG	60.031	559	hypothetical protein MIMGU_mgv1a015066mg [Erythranthe guttata]
8	Perilla-1-3a_pTriplEx2-seq_L05	CT()	CCCAAATTCACATCCACTGA	59.343	AACAACTGACATGGCCTTCC	59.973	185	PREDICTED: uncharacterized protein LOC105160440 [Sesamum indicum]
9	Perilla-1-3a_pTriplEx2-seq_L15	TC()	CAGTTTTAACTTCGCCTCGC	60.018	CACTCGCAAAAAGGGGTAAG	59.741	619	PREDICTED: annexin D5 [Sesamum indicum]
10	Perilla-2-1a_pTriplEx2-seq_A19	GGA()	GCTCCTCGCAGTAACTTTGG	60.015	TCATCTCTTGCTCTGTTTCCA	58.583	107	hypothetical protein MIMGU_mgv1a016040mg [Erythranthe guttata]
11	Perilla-2-2a_pTriplEx2-seq_A06	CT()	CATTGGCCTTAAACTTCGGA	60.067	ATAAATGTGGATTGGGGCAA	60.016	341	hypothetical protein MIMGU_mgv1a020048mg [Erythranthe guttata]
12	Perilla-2-2a_pTriplEx2-seq_C18	AG()	GGGGGATCATTTCCAGTCTT	60.133	GTGCCCACTGGTTCTTTGTT	60.012	404	hypothetical protein MIMGU_mgv1a012334mg [Erythranthe guttata]
13	Perilla-3-2a_pTriplEx2-seq_E14	GATGACGATGAT()	CTTTCCAACCCTCCGAATTT	60.291	CGACGCCTGTCTCATCTACA	60.008	522	NAC transcription factor 1 [Salvia miltiorrhiza]
14	Perilla-3-2a_pTriplEx2-seq_O22	GA()	GGGGATATGTTATGTTGCTTGTT	59.179	TCGCCGTACTTGATCCCTAC	60.096	514	PREDICTED: uncharacterized protein LOC105169169 isoform X1 [Sesamum indicum]
15	Perilla-3-3a_pTriplEx2-seq_B03	CT()	CGAGTGTGTTCGTATGGGTG	60.025	AACGCGTACGGAACAGAGAC	60.321	184	hypothetical protein MIMGU_mgv1a014836mg [Erythranthe guttata]
16	Perilla-3-3a_pTriplEx2-seq_B23	TCCTCTTCCTCTCC()	TAGTGTCGAAGCTCAATGGC	59.028	TGACCAGCATCAGCTTTCAC	59.992	662	PREDICTED: chloroplast stem-loop binding protein of 41 kDa a, chloroplastic [Sesamum indicum]
17	Perilla-3-3a_pTriplEx2-seq_F11	GAG()	GAAAGACTGGTTGGCTCTGG	59.844	ATCCAAAATTCGTCCTGTGC	59.939	381	hypothetical protein MIMGU_mgv1a011207mg [Erythranthe guttata]
18	Perilla-3-3a_pTriplEx2-seq_P03	GA()	AAAGCTGTTTGCCCTTGCTA	60.018	CTCAAATGGAGTCACGCAGA	59.984	284	hypothetical protein MIMGU_mgv1a016830mg [Erythranthe guttata]