De Novo Assembly of the Pea (Pisum sativum L.) Nodule Transcriptome

Table 2

Pea nodule transcripts corresponding to known symbiotic genes of Medicago truncatula and Lotus japonicus.

Gene	Accession number (M. truncatula or L. japonicus)	Contig in “Nodules”	Forward primer, 5′-3′	Reverse primer, 5′-3′	Identity % (CDS)
EFD	EU251063.1	TR2716|c0_g1_i1	ACCTTCACTTCACTTCACTTAAG	GGTGTCATGGAGAAATGCTACA	88%
NSP1	AJ972478.1	TR20452|c0_g1_i2	AATGATCCAAGAACACTACTAACC	CAGCTCTCTTAATCACAGACAT	88%
VPY	GQ423209.1	TR12524|c0_g2_i1	ACCATCATAAACCAAACTGTTGC	TCCAAATCACACTCACAACTCC	93%
Sen1	AB573230.1	TR29546|c0_g1_i1	TAAACAGATCAATCAAGCATTCATG	ATTGGTTCAACATGAAGTATACG	76%

% identity to the L. japonicus sequence; for others, % identity to M. truncatula sequences is given.