|
| Name | Sequence | Amplicon size (bp) | Details |
|
| RT-PCR for validation of Alu-exonized transcript and identification of TSEN54 alternative transcripts |
| TSEN54 RT_S1 | 5′-GGT TCC GGG AAG ATG TCA AC-3′ | 236 | |
| TSEN54 RT_S2 | 5′-GCC AAG GTG GGC AGA TCA-3′ | 404 | |
| TSEN54 RT_AS | 5′-CAG CTG GGC ACC TCC ATC-3′ | | |
|
| qRT-PCR for the expression features of TSEN54 transcripts |
| TSEN54 qRT_S1 | 5′-CAG CTG TGG TCC TTC AGC-3′ | 198 | Targeted to original transcript |
| TSEN54 qRT_AS1 | 5′-GGA CAG GCT CAT CAA ATC CAC-3′ |
| TSEN54 qRT_S2 | 5′-TCA TGC CAC TAT ACT CCA GCC-3′ | 213 | Targeted to Alu-exonized transcript in primate |
| TSEN54 qRT_AS2 | 5′-CAG CTG GGC ACC TCC ATC-3′ |
| TSEN54 qRT_S2-1 | 5′-GCT AAA TCT GGC CGT CCT AA-3′ | 112 | Targeted to Alu-exonized transcript in human |
| TSEN54 qRT_AS2-1 | 5′-AGC ACA GAG ATA TGC TGA AGG A-3′ |
|
| qRT-PCR reference genes |
| ARL1 qRT_S | 5′-AGA CAG TTG TGA CCG AGA CC-3′ | 136 | Cynomolgus monkey |
| ARL1 qRT_AS | 5′-TGA GGA AGT CAT GGC CTG TT-3′ |
| MRFAP1 qRT_S | 5′-GCG GAT AGA GAA GAG CGA GT-3′ | 82 |
| MRFAP1 qRT_AS | 5′-AGC CAA TCT CCA CCA GTT GA-3′ |
| ARFGAP2 qRT_S | 5′-GCG TCC ATC TGA GCT TCA TC-3′ | 135 |
| ARFGAP2 qRT_AS | 5′-CAT CAT TGG CTG TGC ATC CA-3′ |
| RPL32 qRT_S | 5′-CAA CAT TGG TTA TGG AAG CAA CA-3′ | 80 | Rhesus monkey and human |
| RPL32 qRT_AS | 5′-TGA CGT TGT GGA CCA GGA ACT-3′ |
| RPL13A qRT_S | 5′-CCT GGA GGA GAA GAG GAA AGA GA-3′ | 126 | Rhesus monkey |
| RPL13A qRT_AS | 5′-TTG AGG ACC TCT GTG TAT TTG TCA A-3′ |
| HMBS qRT_S | 5′-ACC AAG GAG CTT GAA CAT GC-3′ | 145 | Human |
| HMBS qRT_AS | 5′-GAA AGA CAA CAG CAT CAT GAG-3′ |
| GAPDH qRT_S | 5′-GAA ATC CCA TCA CCA TCT TCC AGG-3′ | 120 |
| GAPDH qRT_AS | 5′-GAG CCC CAG CCT TCT CCA TG-3′ |
|
| Genomic DNA PCR for evolutionary analysis of AluSx insertion |
| TSEN54 GS | 5′-ATG GGA ATG CGG TAG ATT GT-3′ | 508 | |
| TSEN54 GAS | 5′-AGG GGA GTC ACA TTT CTC AGT C-3′ |
|
