Performance Characterization and Validation of Saliva as an Alternative Specimen Source for Detecting Hereditary Breast Cancer Mutations by Next Generation Sequencing

Table 1

Concentration of DNA samples extracted from paired blood and saliva as quantified by Qubit and spectrophotometer. Sample tracking Ion Xpress barcodes and concentrations of Ampliseq libraries generated by each DNA sample.

Sample ID	DNA Qubit quantification		DNA spectrophotometer quantification		OD260/OD280	Ion Xpress barcode (s)	Library concentration (ng/mL)
	Concentration (ng/µL)	Total yield (µg)	Concentration (ng/L)	Total yield (g)
1601074001Saliva	20.0	1.0	38.4	1.9	1.808	13	148
1601074001Blood	97.4	4.8	148.5	7.4	1.724	14	191
1601074002Saliva	18.3	0.9	29.4	1.4	1.942	11	528
1601074002Blood	110.0	5.5	166.6	8.3	1.744	12	139
1601074003Saliva	10.9	0.5	27.2	1.3	1.865	11	290
1601074003Blood	57.4	2.8	291.6	14.5	1.913	10	476
1601074004Saliva	10.8	0.5	15.8	0.7	1.945	13	185
1601074004 Blood	93.2	4.6	105.6	5.2	1.847	12	75.4
1601074005Saliva	14.2	0.7	19.0	0.9	1.895	9	77.4
1601074005 Blood	78.2	3.9	189.2	9.4	1.891	8	69.2
1601074006 Saliva	20.8	1.0	37.7	1.8	1.843	7	50
1601074006Blood	114.0	5.7	140.7	7	1.88	6	58.8
Mean (blood)	91.7	4.6	173.7	8.6	1.88		168.2
Mean (saliva)	15.8	0.8	27.9	1.3	1.83		213
p value: blood versus saliva (T test)	>0.001		>0.01