International Journal of Genomics / 2016 / Article / Tab 1

Research Article

Performance Characterization and Validation of Saliva as an Alternative Specimen Source for Detecting Hereditary Breast Cancer Mutations by Next Generation Sequencing

Table 1

Concentration of DNA samples extracted from paired blood and saliva as quantified by Qubit and spectrophotometer. Sample tracking Ion Xpress barcodes and concentrations of Ampliseq libraries generated by each DNA sample.

Sample IDDNA Qubit quantificationDNA spectrophotometer quantificationOD260/OD280Ion Xpress barcode (s)Library concentration (ng/mL)
Concentration (ng/µL) Total yield (µg) Concentration (ng/L) Total yield (g)

1601074001Saliva20.01.038.41.91.80813148
1601074001Blood97.44.8148.57.41.72414191
1601074002Saliva18.30.929.41.41.94211528
1601074002Blood110.05.5166.68.31.74412139
1601074003Saliva10.90.527.21.31.86511290
1601074003Blood57.42.8291.614.51.91310476
1601074004Saliva10.80.515.80.71.94513185
1601074004 Blood93.24.6105.65.21.8471275.4
1601074005Saliva14.20.719.00.91.895977.4
1601074005 Blood78.23.9189.29.41.891869.2
1601074006 Saliva20.81.037.71.81.843750
1601074006Blood114.05.7140.771.88658.8
Mean (blood)91.74.6173.78.61.88168.2
Mean (saliva)15.80.827.91.31.83213
p value: blood versus saliva (T test)>0.001>0.01

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