Gene Expression Analysis of Alfalfa Seedlings Response to Acid-Aluminum

Table 2

Differentially expressed genes across all treatments.

	B versus A	C versus A	D versus A	C versus B	D versus B	D versus C
Downregulated	1126	340	566	735	1111	595
Upregulated	1379	1037	912	1381	1893	304
Total	4146

All of the genes mapped to the reference sequence and genome sequences were examined for differences in expression across the different libraries. Numbers of differentially expressed genes were analyzed across sense transcripts using a threshold value FC ≥ 2. A, B, C, and D represent germinated seeds treated with 0 (pH 6.0), 0 (pH 4.5), 0.8 (pH 4.5), and 3.2 (pH 4.5) mM AlCl₃ solution for 60 h, respectively.