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International Journal of Genomics
Volume 2016, Article ID 3034756, 13 pages
Research Article

Genetic Characterization and Comparative Genome Analysis of Brucella melitensis Isolates from India

1National Institute of Animal Biotechnology, Hyderabad 500049, India
2Ella Foundation, Genome Valley, Turkapally, Shameerpet, Hyderabad 500078, India
3Central Institute for Research on Goats, Makhdoom, Mathura 281122, India

Received 11 April 2016; Revised 26 May 2016; Accepted 29 May 2016

Academic Editor: Shen Liang Chen

Copyright © 2016 Sarwar Azam et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Brucellosis is the most frequent zoonotic disease worldwide, with over 500,000 new human infections every year. Brucella melitensis, the most virulent species in humans, primarily affects goats and the zoonotic transmission occurs by ingestion of unpasteurized milk products or through direct contact with fetal tissues. Brucellosis is endemic in India but no information is available on population structure and genetic diversity of Brucella spp. in India. We performed multilocus sequence typing of four B. melitensis strains isolated from naturally infected goats from India. For more detailed genetic characterization, we carried out whole genome sequencing and comparative genome analysis of one of the B. melitensis isolates, Bm IND1. Genome analysis identified 141 unique SNPs, 78 VNTRs, 51 Indels, and 2 putative prophage integrations in the Bm IND1 genome. Our data may help to develop improved epidemiological typing tools and efficient preventive strategies to control brucellosis.