Genome-Wide Analysis of Genes Encoding Methionine-Rich Proteins in Arabidopsis and Soybean Suggesting Their Roles in the Adaptation of Plants to Abiotic Stress

Table 1

Genes encoding AtMRPs whose expression levels were responsive to both drought and high salinity.

Number	Locus IDs	Met (%)	Length (a.a.)	Drought versus untreated^1		Salinity versus untreated^2		Gene descriptions
				Fold change^3	-value	Fold change^3	-value
1	AT1G32560	6.02	134	135.33	0.002	3.31	0.005	LEA group 1 domain-containing protein
2	AT1G33860	8.55	153	2.37	0.092	2.16	0.003	Unknown protein
3	AT3G55240	6.12	95	−60.29	0.007	−26.88	0.001	Overexpression leads to pseudo-etiolation in light phenotype
4	AT3G59900	6.20	130	10.70	0.011	−2.57	0.015	(ARGOS); unknown protein [AT3G59900.1]
5	AT3G62090	6.38	346	64.56	0.020	2.28	0.002	PHYTOCHROME INTERACTING FACTOR 3-LIKE 2
6	AT4G12334	6.25	113	−9.79	0.003	−3.04	0.005	Pseudogene of cytochrome P450 family protein
7	AT4G33467	8.91	102	337.51	0.002	6.16	0.023	Unknown protein [AT4G33467.1]
8	AT4G34590	6.33	159	8.26	0.004	3.27	0.002	GBF6 (A. thaliana BASIC LEUCINE-ZIPPER 11)
9	AT5G42325	6.03	233	2.70	0.028	2.45	0.049	Transcription elongation factor-related
10	AT5G67390	7.43	176	−4.17	0.015	−4.15	0.001	Similar to unknown proteins (TAIR:AT1G69360.1)

Two-week-old plants were transferred to soil and allowed to grow for an additional week; the plants were then withheld from watering for 10 days. After 10 d of water with holding, rosette leaves were collected from both well watered and drought-stressed plants in three biological replicates, frozen in liquid nitrogen, and stored at −80°C until used for RNA extraction [35]. 10-day-old plants grown on GM media were transferred onto 0.5 × MS plates without sucrose, containing either 0 mM (control) or 200 mM NaCl, and maintained for a period of 24 h. Three independent experiments were performed for each condition. The samples were collected as three biological replicates (10 plants/replicate), frozen in liquid nitrogen, and stored at −80°C until used for RNA extraction [34]. Stress responsive AtMRPs were defined as genes encoding MRP whose expression levels were induced or repressed 2-fold or more with an FDR corrected value of less than 0.05.