Use of Posttranscription Gene Silencing in Squash to Induce Resistance against the Egyptian Isolate of the Squash Leaf Curl Virus

Figure 8

siRNA-mediated silencing of Rep/TrAP and BC1 was assessed using ΔΔCt method to determine relative gene expression from qPCR data using 18s rRNA as an endogenous reference gene. The cells exhibited siRNA of Rep/TrAP reduced the mRNA level by 97%, while siRNA of BC1 reduced mRNA level by 79% compared with mRNA of control samples which fully expressed the squash leaf curl virus genes.