Use of Posttranscription Gene Silencing in Squash to Induce Resistance against the Egyptian Isolate of the Squash Leaf Curl Virus

Table 2

Quantitative real-time PCR estimation of viral expression levels for SqLCV in plants infiltrated by pFGC-Rep/TrAP and pFGC-BC1.

	Rep/TrAP gene				BC1 gene
	Ct of control	Ct of control reference	Ct of treatment	Ct of treatment reference	Ct of control	Ct of control reference	Ct of treatment	Ct of treatment reference
Replica 1	24.26	16.47	29.28	16.28	27.07	16.92	29.41	16.64
Replica 2	24.55	16.00	29.39	16.19	27.94	16.77	29.89	16.7
Replica 3	24.07	16.38	29.87	15.98	27.45	16.89	29.44	16.82
Average	24.29	16.28	29.51	16.15	27.48	16.86	29.58	16.72
	8.01		13.01		10.63		12.86
	5				2.23
Fold of change =	0.03125				0.213
Silencing % =	97%				79%

Cts = Ct values of sample; Ctr = Ct value of housekeeping gene for the same sample; Ctc = Ct values of control; Ctt = Ct values of treatment.