RNA Sequencing of Formalin-Fixed, Paraffin-Embedded Specimens for Gene Expression Quantification and Data Mining

Figure 1

(a) Unsupervised cluster using all detected RNAs. Samples were clustered first by replicates then by rRNA depletion method. (b) Pairwise Spearman correlation heatmap between all samples. Ribo-Zero produced higher correlation between repeats than RNase H. The samples RN4 and RN4r produce low correlations with other samples compared to other random pairs. This could be the result of variation in the sample or variation introduced by the RNase H kit.

(a)

(b)