RNA Sequencing of Formalin-Fixed, Paraffin-Embedded Specimens for Gene Expression Quantification and Data Mining

Figure 5

Callable site is defined as a genomic position with depth coverage ≥ 20. The number of callable sites indicates the number of genomic positions that are suitable for SNV inference. RNase H had substantially more callable sites than Ribo-Zero. The percentage of difference in callable site is significantly more than the percentage of difference in number of total reads sequenced by the two kits. -axis is plotted in log₁₀ scale.