RNA Sequencing of Formalin-Fixed, Paraffin-Embedded Specimens for Gene Expression Quantification and Data Mining

Table 1

Sample description and alignment statistics.

ID	Library	Raw data			Alignment
		Total reads	BQ	GC	CR	Non-CR	CR MQ	Non-CR MQ
1	Ribo-Zero	17.8 M	31	71.4%	27.7%	72.3%	32	47
2	Ribo-Zero	16.1 M	30	76.8%	31.4%	68.6%	23	47
3	Ribo-Zero	16.8 M	31	70.7%	31.6%	68.4%	28	46
4	Ribo-Zero	14.0 M	31	72.3%	46.0%	54.0%	34	47
1	Ribo-Zero	16.1 M	31	70.4%	27.4%	72.6%	31	47
2	Ribo-Zero	14.9 M	31	75.4%	37.9%	62.1%	21	47
3	Ribo-Zero	17.6 M	31	71.1%	29.8%	70.2%	29	47
4	Ribo-Zero	15.6 M	31	70.0%	46.1%	53.9%	36	47
1	RNase H	20.2 M	35	51.6%	79.9%	20.1%	46	33
2	RNase H	20.5 M	36	39.8%	42.6%	57.4%	45	41
3	RNase H	20.4 M	35	51.6%	78.9%	21.1%	45	33
4	RNase H	21.4 M	35	48.6%	58.0%	42.0%	45	37
1	RNase H	22.1 M	35	52.5%	80.3%	19.7%	46	35
2	RNase H	22.4 M	34	55.1%	74.7%	25.3%	44	33
3	RNase H	20.6 M	35	52.0%	78.5%	21.5%	45	31
4	RNase H	24.0 M	34	53.6%	80.1%	19.9%	45	30

CR: coding region; BQ: base quality; MQ: mapping quality; GC: GC content.