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International Journal of Genomics
Volume 2017, Article ID 1032846, 14 pages
https://doi.org/10.1155/2017/1032846
Research Article

Genomic Survey, Characterization, and Expression Profile Analysis of the SBP Genes in Pineapple (Ananas comosus L.)

1Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology; Key Lab of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education; State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops; Center for Genomics and Biotechnology; College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province 350002, China
2College of Crop Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
3College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
4Abasyn University, Peshawar, 25000 KPK, Pakistan

Correspondence should be addressed to Yuan Qin; nc.ude.ufaf@niqnauy

Received 17 May 2017; Revised 30 July 2017; Accepted 6 September 2017; Published 29 September 2017

Academic Editor: Ferenc Olasz

Copyright © 2017 Hina Ali et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Gene expression is regulated by transcription factors, which play many significant developmental processes. SQUAMOSA promoter-binding proteins (SBP) perform a variety of regulatory functions in leaf, flower, and fruit development, plant architecture, and sporogenesis. 16 SBP genes were identified in pineapple and were divided into four groups on basis of phylogenetic analysis. Five paralogs in pineapple for SBP genes were identified with Ka/Ks ratio varied from 0.20 for AcSBP14 and AcSBP15 to 0.36 for AcSBP6 and AcSBP16, respectively. 16 SBP genes were located on 12 chromosomes out of 25 pineapple chromosomes with highly conserved protein sequence structures. The isoionic points of SBP ranged from 6.05 to 9.57, while molecular weight varied from 22.7 to 121.9 kD. Expression profiles of SBP genes revealed that AcSBP7 and AcSBP15 (leaf), AcSBP13, AcSBP12, AcSBP8, AcSBP16, AcSBP9, and AcSBP11 (sepal), AcSBP6, AcSBP4, and AcSBP10 (stamen), AcSBP14, AcSBP1, and AcSBP5 (fruit) while the rest of genes showed low expression in studied tissues. Four genes, that is, AcSBP11, AcSBP6, AcSBP4, and AcSBP12, were highly expressed at 4°C, while AcSBP16 were upregulated at 45°C. RNA-Seq was validated through qRT-PCR for some genes. Salt stress-induced expression of two genes, that is, AcSBP7 and AcSBP14, while in drought stress, AcSBP12 and AcSBP15 were highly expressed. Our study lays a foundation for further gene function and expression studies of SBP genes in pineapple.