Case Study of Somaclonal Variation in Resistance Genes Mlo and Pme3 in Flaxseed (Linum usitatissimum L.) Induced by Nanoparticles

Table 1

Sequence characteristics of primers developed in this study. PCR and sequencing primers were designed for amplification the gene regions of Pme3 and Mlo.

Primer ID	Primer sequence (5′-3′)	Primer melting temperature	Product length	Accession number
LuMlo-F	CCGATTCTATGGTTTTTTGCAGTC	54°C	226 bp	AJ005341.1
LuMlo-R	GGCAGAAATGGATGAGGAAGAGA	56°C
LuMlo-S	AAGCTACAAGTGATCATAAC	47°C	220 bp
LuPme3-F	CCGCTTGAACATAGAAAAACAGCT	56°C	573 bp	AF308812.1
LuPme3-R	TCTTTTTTTTCACGTGGCGACT	55°C
LuPme3-S	TTTAGCCCAAAATTGAG	44°C	560 bp