Characteristics and Validation Techniques for PCA-Based Gene-Expression Signatures

Figure 1

Conceptual view of how a good PCA-based gene signature should behave. The genes in a signature should be coherently expressed within the data (a). A signature should represent a single biological effect (b). A signature should represent unique and distinct signal within the data (c). A signature should represent the same biology as a known reference dataset (d).

(a)

(b)

(c)

(d)