Characterization and Expression Analysis of Common Bean Histone Deacetylase 6 during Development and Cold Stress Response

Figure 3

Validation of relative expression level of HDA6 by qPCR using leaf tissues at different developmental stages of common bean. (a) Relative expression of HDA6 gene in leaves at the seedling, flowering, and pod developmental stages of common bean. Three biological and three technical replicates were used for the analysis. The alphabets in the figure show that the expressions of HDA6 in the leaf tissue at the different developmental stages are statistically significant at . (b) Diagram illustrating the common bean specific HDA6 polyclonal antibody raised for protein validation. The 15 amino acids’ peptide indicated in faded lines with red arrow is one of the three peptides selected for the epitope. (c) Western blot analysis using common bean specific HDA6 antibody. The figure further validates the role of HDA6 in common bean development.

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