Characterization and Expression Analysis of Common Bean Histone Deacetylase 6 during Development and Cold Stress Response

Figure 4

Spatial expression profile of HDA6 in five tissues of common bean. (a) Traditional PCR was conducted using HDA6 primers designed for qPCR. Each of the cDNA samples were normalized to 200 ng/μL. Regular PCR was conducted using HDA6 real-time primers designed for qPCR. The cons7 primer which is a constitutive gene was used as a control. (b) Determination of the relative expression of HDA6 transcript in different tissues of common bean by qPCR. The alphabets indicate significant difference between common bean tissues at . (c) Western blot analysis using HDA6 specific antibody with proteins isolated from empty pET vector (1) as a negative control, (2) leaf, (3) flower, and (4) pod.

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