Characterization and Expression Analysis of Common Bean Histone Deacetylase 6 during Development and Cold Stress Response

Figure 5

Expression analysis of HDA6 after subjecting common bean plants to cold treatment at 4°C at different time points. Leaf tissues were collected from control and cold treated plants 24 and 72 hours after treatment. (a) PCR performed after RT-PCR of samples using HDA6 and Actin primers. (b) qPCR to determine relative expression of HDA6 during cold stress. Three biological and three technical replicates were used for the analysis. Data were analyzed by analysis of variance (ANOVA) using [SAS] statistical software. Differences between treatments were assessed by Tukey’s Studentized Range (HSD) Test for experiment. There was significant difference between control and cold treated samples at . (c) Coomassie stained gel of proteins extracted from control and cold treated samples. (d) Western blot analysis using HDA6 specific antibody with proteins isolated from control and cold treated samples.

(a)

(b)

(c)

(d)