Characterization and Expression Analysis of Common Bean Histone Deacetylase 6 during Development and Cold Stress Response

Figure 6

Expression analysis of DREB1A, a cold responsive gene after subjecting common bean plants to cold treatment at 4°C at different time points. Leaf tissues were collected from control and cold treated plants 24 and 72 hours after treatment. (a) cDNA PCR performed after RT-PCR of samples using DREB 1A and Actin primers. (b) qPCR to determine relative expression of DREB 1A during cold stress. Three biological and three technical replicates were used for the analysis. Data were analyzed by analysis of variance (ANOVA) using [SAS] statistical software. Differences between treatments were assessed by Tukey’s Studentized Range (HSD) Test for experiment. The different letters A, B, and C indicate significant difference in gene expression between times of cold treatment at . Values with the same letters are not statistically different.

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