Development of a New Marker System for Identification of Spirodela polyrhiza and Landoltia punctata

Table 2

Three pairs of primers designed and used well for duckweed ecotype identification. “T (°C)” represents the degenerate temperature.

Primers	Primer sequences ()	T (°C)	PCR product length range
SC09/10	TTAGTATTGTGCGACATTCG	52	170–256 bp
	TTTCTTTGATTTGAACTCCC
SC19/20	GCGTTCTGTTTCTTTACCTA	53	212–284 bp
	CGGAGTAGAGCAGTTTGG
SC35/36	ACCCTGGAGCATACCTTG	53	224–298 bp
	AGGATTAGGAATGGGCGT