The miRNA Pull Out Assay as a Method to Validate the miR-28-5p Targets Identified in Other Tumor Contexts in Prostate Cancer
Effects of miR-28-5p reexpression on DU-145 cells. Cell survival (a) and cell cycle (b) in DU-145 cells after miR-28-5p reexpression. Apoptosis analysis measured with both annexin assay (c) and western blot of PARP-1 and cleaved PARP-1 (d) in miR-28-5p-transfected DU-145 cells. and , unpaired t-test.
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