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International Journal of Genomics
Volume 2017, Article ID 6347874, 9 pages
https://doi.org/10.1155/2017/6347874
Review Article

Aquatic Plant Genomics: Advances, Applications, and Prospects

1The State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei 430072, China
2University of Chinese Academy of Sciences, Beijing 100049, China

Correspondence should be addressed to Hongwei Hou; nc.ca.bhi@whuoh

Received 15 December 2016; Revised 11 July 2017; Accepted 30 July 2017; Published 16 August 2017

Academic Editor: Graziano Pesole

Copyright © 2017 Shiqi Hu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. A. G. Initiative, “Analysis of the genome sequence of the flowering plant Arabidopsis thaliana,” Nature, vol. 408, no. 6814, pp. 796–815, 2000. View at Publisher · View at Google Scholar · View at Scopus
  2. S. A. Goff, D. Ricke, T. H. Lan et al., “A draft sequence of the rice genome (Oryza sativaL. ssp. japonica),” Science, vol. 296, no. 5565, pp. 92–100, 2002. View at Google Scholar
  3. Tomato Genome Consortium, “The tomato genome sequence provides insights into fleshy fruit evolution,” Nature, vol. 485, no. 7400, pp. 635–641, 2012. View at Publisher · View at Google Scholar · View at Scopus
  4. K. F. Mayer, J. Rogers, J. Doležel et al., “A chromsome-based draft sequence of the hexaploid bread wheat (triticumaestivum) genome,” Science, vol. 345, no. 6194, 2014. View at Publisher · View at Google Scholar · View at Scopus
  5. C. Qin, C. Yu, Y. Shen et al., “Whole-genome sequencing of cultivated and wild peppers provides insights into capsicum domestication and specialization,” Proceedings of the National Academy of Sciences of the United States of America, vol. 111, no. 14, pp. 5135–5140, 2014. View at Publisher · View at Google Scholar · View at Scopus
  6. P. Schnable, D. Ware, R. Fulton et al., “The b73 maize genome: complexity, diversity, and dynamics,” Science, vol. 326, no. 5956, pp. 1112–1115, 2009. View at Publisher · View at Google Scholar · View at Scopus
  7. X. W. Zou, “Development of aquatic plants at home and abroad (in Chinese),” China Flowers & Horticulture, vol. 15, pp. 10–12, 2005. View at Google Scholar
  8. B. J. Pollux, M. D. Jong, A. Steegh, E. Verbruggen, J. M. Groenendael, and N. J. Ouborg, “Reproductive strategy, clonal structure and genetic diversity in populations of the aquatic macrophyte Sparganium emersum in river systems,” Molecular Ecology, vol. 16, no. 2, pp. 313–325, 2007. View at Publisher · View at Google Scholar · View at Scopus
  9. J. Martinez-Garrido, M. Gonzalez-Wanguemert, and E. A. Serrao, “New highly polymorphic microsatellite markers for the aquatic angiosperm Ruppia cirrhosa reveal population diversity and differentiation,” Genome, vol. 57, no. 1, pp. 57–59, 2014. View at Publisher · View at Google Scholar · View at Scopus
  10. A. Imanishi, S. Kaneko, Y. Isagi, J. Imanishi, Y. Natuhara, and Y. Morimoto, “Development of microsatellite markers for Euryale ferox (Nymphaeaceae), an endangered aquatic plant species in Japan,” American Journal of Botany, vol. 98, no. 8, pp. 233–235, 2011. View at Publisher · View at Google Scholar · View at Scopus
  11. R. Uesugi, N. Tani, K. Goka, J. Nishihiro, Y. Tsumura, and I. Washitani, “Isolation and characterization of highly polymorphic microsatellites in the aquatic plant, Nymphoides peltata (Menyanthaceae),” Molecular Ecology Notes, vol. 5, no. 2, pp. 343–345, 2005. View at Google Scholar
  12. Y. Y. Liao, X. L. Yue, Y. H. Guo, W. R. Gituru, Q. F. Wang, and J. M. Chen, “Genotypic diversity and genetic structure of populations of the distylous aquatic plant Nymphoides peltata (Menyanthaceae) in China,” Journal of Systematics and Evolution, vol. 51, no. 5, pp. 536–544, 2013. View at Google Scholar
  13. Y. Kameyama and M. Ohara, “Predominance of clonal reproduction, but recombinant origins of new genotypes in the free-floating aquatic bladderwort Uricularia australis f. tenuicaulis (Lentibulariaceae),” Journal of Plant Research, vol. 119, no. 4, pp. 357–362, 2006. View at Publisher · View at Google Scholar · View at Scopus
  14. K. Koga, Y. Kadono, and H. Setoguchi, “The genetic structure of populations of the vulnerable aquatic macrophyte Ranunculus nipponicus (Ranunculaceae),” Journal of Plant Research, vol. 120, no. 2, pp. 167–174, 2007. View at Publisher · View at Google Scholar · View at Scopus
  15. Y. Y. Yuan, Q. F. Wang, and J. M. Chen, “Development of SSR markers in aquatic plant Nymphoides peltata (Menyanthaceae) based on information from transcriptome sequencing (in Chinese),” Plant Sclence Journal, vol. 31, no. 5, pp. 485–492, 2013. View at Google Scholar
  16. B. Wang, W. Li, and J. Wang, “Genetic diversity of Alternanthera philoxeroides in China,” Aquatic Botany, vol. 81, no. 3, pp. 277–283, 2005. View at Publisher · View at Google Scholar · View at Scopus
  17. H. Kumar, P. Priya, N. Singh et al., “RAPD and ISSR marker-based comparative evaluation of genetic diversity among Indian germplasms of Euryale ferox: an aquatic food plant,” Applied Biochemistry and Biotechnology, vol. 180, no. 7, pp. 1345–1360, 2016. View at Publisher · View at Google Scholar · View at Scopus
  18. T. D. Barbosa, R. J. Trad, M. M. Bajay, and M. C. Amaral, “Microsatellite markers isolated from Cabomba aquatica s.L. (Cabombaceae) from an enriched genomic library,” Applications in Plant Sciences, vol. 3, no. 11, 2015. View at Publisher · View at Google Scholar · View at Scopus
  19. J. Hu, L. Pan, H. Liu et al., “Comparative analysis of genetic diversity in sacred lotus (Nelumbo nucifera Gaertn.) using AFLP and SSR markers,” Molecular Biology Reports, vol. 39, no. 4, pp. 3637–3647, 2011. View at Publisher · View at Google Scholar · View at Scopus
  20. L. Y. Chen, J. M. Chen, R. W. Gituru, and Q. F. Wang, “Generic phylogeny, historical biogeography and character evolution of the cosmopolitan aquatic plant family Hydrocharitaceae,” BMC Evolutionary Biology, vol. 12, no. 1, pp. 1–12, 2012. View at Publisher · View at Google Scholar · View at Scopus
  21. F. Y. Ellmouni, M. A. Karam, R. M. Ali, and D. C. Albach, “Molecular and morphometric analysis of Veronica L. section Beccabunga (Hill) Dumort,” Aquatic Botany, vol. 136, pp. 95–111, 2017. View at Publisher · View at Google Scholar
  22. J. Zalewska-Gałosz and M. Ronikier, “Potamogeton ×maëmetsiae: a new hybrid between linear-leaved pondweeds from central Europe,” Preslia -Praha, vol. 83, no. 3, pp. 259–273, 2011. View at Google Scholar
  23. J. B. Whittall and S. A. Hodges, “Cryptic species in an endangered pondweed community (Potamogeton, potamogetonaceae) revealed by AFLP markers,” American Journal of Botany, vol. 91, no. 12, pp. 2022–2029, 2004. View at Publisher · View at Google Scholar · View at Scopus
  24. E. Ibarra-Laclette, E. Lyons, G. Hernández-Guzmán et al., “Architecture and evolution of a minute plant genome,” Nature, vol. 498, no. 7452, pp. 94–98, 2013. View at Publisher · View at Google Scholar · View at Scopus
  25. L. Carretero-Paulet, T. H. Chang, P. Librado et al., “Genome-wide analysis of adaptive molecular evolution in the carnivorous plant Utricularia gibba,” Genome Biology & Evolution, vol. 7, no. 2, p. 444, 2015. View at Publisher · View at Google Scholar · View at Scopus
  26. W. Wang and J. Messing, “Status of duckweed genomics and transcriptomics,” Plant Biology (Stuttgart, Germany), vol. 17, Supplement 1, pp. 10–15, 2015. View at Publisher · View at Google Scholar · View at Scopus
  27. M. Matsuzaki, O. Misumi, T. Shin-I et al., “Genome sequence of the ultrasmall unicellular red alga Cyanidioschyzon merolae 10D,” Nature, vol. 428, no. 6983, pp. 653–657, 2004. View at Publisher · View at Google Scholar · View at Scopus
  28. S. S. Merchant, S. E. Prochnik, O. Vallon et al., “The Chlamydomonas genome reveals the evolution of key animal and plant functions,” Science, vol. 318, no. 5848, pp. 245–250, 2007. View at Publisher · View at Google Scholar · View at Scopus
  29. E. V. Armbrust, J. A. Berges, C. Bowler et al., “The genome of the diatom Thalassiosira pseudonana: ecology, evolution, and metabolism,” Science, vol. 306, no. 5693, pp. 79–86, 2004. View at Publisher · View at Google Scholar · View at Scopus
  30. A. Montsant, K. Jabbari, U. Maheswari, and C. Bowler, “Comparative genomics of the pennate diatom Phaeodactylum tricornutum,” Plant Physiology, vol. 137, no. 2, pp. 500–513, 2005. View at Publisher · View at Google Scholar · View at Scopus
  31. S. K. Rao, H. Fukayama, J. B. Reiskind, M. Miyao, and G. Bowes, “Identification of C4 responsive genes in the facultative C4 plant Hydrilla verticillata,” Photosynthesis Research, vol. 88, no. 2, pp. 173–183, 2006. View at Publisher · View at Google Scholar · View at Scopus
  32. J. Silverthorne, C. F. Wimpee, T. Yamada, S. A. Rolfe, and E. M. Tobin, “Differential expression of individual genes encoding the small subunit of ribulose-1,5-bisphosphate carboxylase in Lemna gibba,” Plant Molecular Biology, vol. 15, no. 1, pp. 49–58, 1990. View at Google Scholar
  33. J. Silverthorne and E. M. Tobin, “Post-transcriptional regulation of organ-specific expression of individual rbcs mRNAs in Lemna gibba,” Plant Cell, vol. 2, no. 12, pp. 1181–1190, 1990. View at Publisher · View at Google Scholar
  34. D. Wang, S. Z. Xie, J. Yang, and Q. F. Wang, “Molecular characteristics and expression patterns of Rubisco activase, novel alternative splicing variants in a heterophyllous aquatic plant, Sagittaria graminea,” Photosynthetica, vol. 52, no. 1, pp. 83–95, 2014. View at Google Scholar
  35. S. K. Baniwal, K. Bharti, K. Y. Chan et al., “Heat stress response in plants: a complex game with chaperones and more than twenty heat stress transcription factors,” Journal of Biosciences, vol. 29, no. 4, pp. 471–487, 2005. View at Google Scholar
  36. E. Vierling, “The roles of heat shock proteins in plants,” Annual Review of Plant Physiology and Plant Molecular Biology, vol. 42, pp. 579–620, 1991. View at Google Scholar
  37. M. Amano, S. Iida, and K. Kosuge, “Comparative studies of thermotolerance: different modes of heat acclimation between tolerant and intolerant aquatic plants of the genus Potamogeton,” Annals of Botany, vol. 109, no. 2, pp. 443–452, 2012. View at Publisher · View at Google Scholar · View at Scopus
  38. D. E. Salt, R. D. Smith, and I. Raskin, “Phytoremediation,” Annual Review of Plant Biology, vol. 49, no. 49, pp. 643–668, 1998. View at Publisher · View at Google Scholar
  39. O. Keskinkan, M. Z. L. Goksu, M. Basibuyuk, and C. F. Forster, “Heavy metal adsorption properties of a submerged aquatic plant (Ceratophyllum demersum),” Bioresource Technology, vol. 92, no. 2, pp. 197–200, 2004. View at Google Scholar
  40. S. Mishra, R. D. Tripathi, S. Srivastava et al., “Thiol metabolism play significant role during cadmium detoxification by Ceratophyllum demersum, L,” Bioresource Technology, vol. 100, no. 7, pp. 2155–2161, 2009. View at Publisher · View at Google Scholar · View at Scopus
  41. Q. Sun, W. B. Liu, and C. Wang, “Different response of phytochelatins in two aquatic macrophytes exposed to cadmium at environmentally relevant concentrations,” African Journal of Biotechnology, vol. 10, no. 33, pp. 6292–6299, 2011. View at Google Scholar
  42. R. D. Tripathi, R. Singh, P. Tripathi et al., “Arsenic accumulation and tolerance in rootless macrophyte Najas indica are mediated through antioxidants, amino acids and phytochelatins,” Aquatic Toxicology, vol. 157, pp. 70–80, 2014. View at Publisher · View at Google Scholar · View at Scopus
  43. U. N. Rai, R. D. Tripathi, N. K. Singh et al., “Constructed wetland as an ecotechnological tool for pollution treatment for conservation of Ganga river,” Bioresource Technology, vol. 148, no. 11, pp. 535–541, 2013. View at Publisher · View at Google Scholar · View at Scopus
  44. S. Mishra, M. Alfeld, R. Sobotka, E. Andresen, G. Falkenberg, and H. Küpper, “Analysis of sublethal arsenic toxicity to Ceratophyllum demersum: subcellular distribution of arsenic and inhibition of chlorophyll biosynthesis,” Journal of Experimental Botany, vol. 67, no. 15, pp. 4639–4646, 2016. View at Publisher · View at Google Scholar · View at Scopus
  45. S. Mishra, S. Srivastava, R. D. Tripathi, and P. K. Trivedi, “Thiol metabolism and antioxidant systems complement each other during arsenate detoxification in Ceratophyllum demersum L,” Aquatic Toxicology, vol. 86, no. 2, p. 205, 2008. View at Publisher · View at Google Scholar · View at Scopus
  46. S. Mishra, G. Wellenreuther, J. Mattusch, H. J. Stärk, and H. Küpper, “Speciation and distribution of arsenic in the nonhyperaccumulator macrophyte Ceratophyllum demersum,” Plant Physiology, vol. 163, no. 3, pp. 1396–1408, 2013. View at Publisher · View at Google Scholar · View at Scopus
  47. D. Shukla, R. Kesari, S. Mishra et al., “Expression of phytochelatin synthase from aquatic macrophyte Ceratophyllum demersum L. enhances cadmium and arsenic accumulation in tobacco,” Plant Cell Reports, vol. 31, no. 9, pp. 1687–1699, 2012. View at Publisher · View at Google Scholar · View at Scopus
  48. M. Shri, R. Dave, S. Diwedi et al., “Heterologous expression of Ceratophyllum demersum phytochelatin synthase, CdPCS1, in rice leads to lower arsenic accumulation in grain,” Scientific Reports, vol. 4, no. 8, pp. 5784–5784, 2014. View at Publisher · View at Google Scholar · View at Scopus
  49. B. Halliwell and J. M. C. Gutteridge, Free Radicals in Biology and Medicine, Oxford University Press, Oxford, UK, 1999.
  50. T. A. Akhtar, M. A. Lampi, and B. M. Greenberg, “Identification of six differentially expressed genes in response to copper exposure in the aquatic plant Lemna gibba (duckweed),” Environmental Toxicology & Chemistry, vol. 24, no. 7, pp. 1705–1715, 2005. View at Google Scholar
  51. L. Xiao, C. Xi, D. J. Oliver, and C. B. Xiang, “Isolation of a low-sulfur tolerance gene from Eichhornia crassipes, using a functional gene-mining approach,” Planta, vol. 231, no. 1, pp. 211–219, 2009. View at Publisher · View at Google Scholar · View at Scopus
  52. O. Hidalgo, S. Garcia, T. Garnatje et al., “Genome size in aquatic and wetland plants: fitting with the large genome constraint hypothesis with a few relevant exceptions,” Plant Systematics and Evolution, vol. 301, no. 7, pp. 1927–1936, 2015. View at Google Scholar
  53. L. A. Raubeson, R. Peery, T. W. Chumley et al., “Comparative chloroplast genomics: analyses including new sequences from the angiosperms Nuphar advena and Ranunculus macranthus,” BMC Genomics, vol. 8, p. 174, 2007. View at Publisher · View at Google Scholar · View at Scopus
  54. E. L. Peredo, U. M. King, and D. H. Les, “The plastid genome of Najas flexilis: adaptation to submersed environments is accompanied by the complete loss of the NDH complex in an aquatic angiosperm,” PLoS One, vol. 8, no. 7, pp. 88–91, 2013. View at Publisher · View at Google Scholar · View at Scopus
  55. T. Huotari and H. Korpelainen, “Complete chloroplast genome sequence of Elodea canadensis and comparative analyses with other monocot plastid genomes,” Gene, vol. 508, no. 1, pp. 96–105, 2012. View at Publisher · View at Google Scholar · View at Scopus
  56. S. R. Silva, D. G. Pinheiro, E. J. Meer, T. P. Michael, A. M. Varani, and V. F. O. Miranda, “The complete chloroplast genome sequence of the leafy bladderwort, Utricularia foliosa L. (Lentibulariaceae),” Conservation Genetics Resources, vol. 9, no. 2, pp. 213–216, 2016. View at Google Scholar
  57. A. V. Mardanov, N. V. Ravin, B. B. Kuznetsov et al., “Complete sequence of the duckweed (Lemna minor) chloroplast genome: structural organization and phylogenetic relationships to other angiosperms,” Journal of Molecular Evolution, vol. 66, no. 6, pp. 555–564, 2008. View at Publisher · View at Google Scholar · View at Scopus
  58. W. Wang and J. Messing, “High-throughput sequencing of three Lemnoideae (duckweeds) chloroplast genomes from total DNA,” PLoS One, vol. 6, no. 9, 2011. View at Publisher · View at Google Scholar · View at Scopus
  59. M. Eisenstein, “The battle for sequencing supremacy,” Nature Biotechnology, vol. 30, no. 11, pp. 1023–1026, 2012. View at Publisher · View at Google Scholar · View at Scopus
  60. W. Wang, Y. Wu, and J. Messing, “The mitochondrial genome of an aquatic plant, Spirodela polyrhiza,” PLoS One, vol. 7, no. 10, pp. 135–139, 2012. View at Publisher · View at Google Scholar · View at Scopus
  61. A. Cuenca, G. Petersen, and O. Seberg, “The complete sequence of the mitochondrial genome of Butomus umbellatus—a member of an early branching lineage of monocotyledons,” PLoS One, vol. 8, no. 4, pp. 542–542, 2013. View at Publisher · View at Google Scholar · View at Scopus
  62. W. Wang, G. Haberer, H. Gundlach et al., “The Spirodela polyrhiza genome reveals insights into its neotenous reduction fast growth and aquatic lifestyle,” Nature Communications, vol. 5, 2014. View at Publisher · View at Google Scholar · View at Scopus
  63. R. Ming, R. Vanburen, Y. Liu et al., “Genome of the long-living sacred lotus (Nelumbo nucifera, Gaertn.),” Genome Biology, vol. 14, no. 5, pp. 241–251, 2013. View at Publisher · View at Google Scholar · View at Scopus
  64. A. V. Hoeck, N. Horemans, P. Monsieurs, H. X. Cao, H. Vandenhove, and R. Blust, “The first draft genome of the aquatic model plant Lemna minor, opens the route for future stress physiology research and biotechnological applications,” Biotechnology for Biofuels, vol. 8, no. 1, pp. 1–13, 2015. View at Publisher · View at Google Scholar · View at Scopus
  65. J. Barta, J. D. Stone, J. Pech et al., “The transcriptome of Utricularia vulgaris, a rootless plant with minimalist genome, reveals extreme alternative splicing and only moderate sequence similarity with Utricularia gibba,” BMC Plant Biology, vol. 15, no. 1, pp. 1–14, 2015. View at Publisher · View at Google Scholar · View at Scopus
  66. E. Ibarra-Laclette, V. A. Albert, C. A. Péreztorres et al., “Transcriptomics and molecular evolutionary rate analysis of the bladderwort (Utricularia), a carnivorous plant with a minimal genome,” BMC Plant Biology, vol. 11, no. 1, pp. 1–16, 2011. View at Publisher · View at Google Scholar · View at Scopus
  67. L. Y. Chen, S. Y. Zhao, Q. F. Wang, and M. L. Moody, “Transcriptome sequencing of three Ranunculus species (Ranunculaceae) reveals candidate genes in adaptation from terrestrial to aquatic habitats,” Scientific Reports, vol. 5, 2015. View at Publisher · View at Google Scholar · View at Scopus
  68. J. Jeon, S. J. Bong, J. S. Park et al., “De novo transcriptome analysis and glucosinolate profiling in watercress (Nasturtium officinale R. Br.),” BMC Genomics, vol. 18, no. 1, p. 401, 2017. View at Publisher · View at Google Scholar
  69. Q. Jiang, F. Wang, H. W. Tan et al., “De novo transcriptome assembly, gene annotation, marker development, and miRNA potential target genes validation under abiotic stresses in Oenanthe javanica,” Molecular Genetics and Genomics, vol. 290, no. 2, pp. 671–683, 2015. View at Publisher · View at Google Scholar · View at Scopus
  70. Y. Zheng, G. Jagadeeswaran, K. Gowdu et al., “Genome-wide analysis of microRNAs in sacred lotus, Nelumbo nucifera (Gaertn),” Tropical Plant Biology, vol. 6, no. 2-3, pp. 117–130, 2013. View at Google Scholar
  71. L. Pan, X. Wang, J. Jin, X. Yu, and J. Hu, “Bioinformatic identification and expression analysis of Nelumbo nucifera microRNA and their targets,” Applications in Plant Sciences, vol. 3, no. 9, article 1500046, 2015. View at Publisher · View at Google Scholar · View at Scopus
  72. M. Thudi, Y. Li, S. A. Jackson, G. D. May, and R. K. Varshney, “Current state-of-art of sequencing technologies for plant genomics research,” Briefings in Functional Genomics, vol. 11, no. 1, pp. 3–11, 2012. View at Publisher · View at Google Scholar · View at Scopus
  73. Y. G. Kim, J. Cha, and S. Chandrasegaran, “Hybrid restriction enzymes: zinc finger fusions to FokI cleavage domain,” Proceedings of the National Academy of Sciences, vol. 93, no. 3, pp. 1156–1160, 1996. View at Google Scholar
  74. T. Li, S. Huang, W. Z. Jiang et al., “TAL nucleases (TALNs): hybrid proteins composed of TAL effectors and FokI DNA-cleavage domain,” Nucleic Acids Research, vol. 39, no. 1, pp. 359–372, 2011. View at Publisher · View at Google Scholar · View at Scopus
  75. M. M. Mahfouz, L. Li, M. Shamimuzzaman, A. Wibowo, X. Fang, and J. K. Zhu, “De novo-engineered transcription activator-like effector (TALE) hybrid nuclease with novel DNA binding specificity creates double-strand breaks,” Proceedings of the National Academy of Sciences, vol. 108, no. 6, pp. 2623–2628, 2011. View at Publisher · View at Google Scholar · View at Scopus
  76. J. C. Miller, S. Tan, G. Qiao et al., “A TALE nuclease architecture for efficient genome editing,” Nature Biotechnology, vol. 29, no. 2, pp. 143–148, 2011. View at Publisher · View at Google Scholar · View at Scopus
  77. M. Jinek, K. Chylinski, I. Fonfara, M. Hauer, J. A. Doudna, and E. Charpentier, “A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity,” Science, vol. 337, no. 6096, pp. 816–821, 2012. View at Publisher · View at Google Scholar · View at Scopus
  78. M. Gurushidze, G. Hensel, S. Hiekel, S. Schedel, V. Valkov, and J. Kumlehn, “True-breeding targeted gene knock-out in barley using designer TALE-nuclease in haploid cells,” PLoS One, vol. 9, no. 3, article e92046, 2014. View at Publisher · View at Google Scholar · View at Scopus
  79. T. Li, B. Liu, M. H. Spalding, D. P. Weeks, and B. Yang, “High-efficiency TALEN-based gene editing produces disease-resistant rice,” Nature Biotechnology, vol. 30, no. 5, pp. 390–392, 2012. View at Publisher · View at Google Scholar · View at Scopus
  80. T. Wendt, P. B. Holm, C. G. Starker et al., “TAL effector nucleases induce mutations at a pre-selected location in the genome of primary barley transformants,” Plant Molecular Biology, vol. 83, no. 3, pp. 279–285, 2013. View at Publisher · View at Google Scholar · View at Scopus
  81. Z. Feng, B. Zhang, W. Ding et al., “Efficient genome editing in plants using a CRISPR/Cas system,” Cell Research, vol. 23, no. 10, pp. 1229–1232, 2013. View at Publisher · View at Google Scholar · View at Scopus
  82. J. F. Li, J. E. Norville, J. Aach et al., “Multiplex and homologous recombination-mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9,” Nature Biotechnology, vol. 31, no. 8, pp. 688–691, 2013. View at Publisher · View at Google Scholar · View at Scopus
  83. V. Nekrasov, B. Staskawicz, and D. Weigel, “Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease,” Nature Biotechnology, vol. 31, no. 8, pp. 691–693, 2013. View at Publisher · View at Google Scholar · View at Scopus
  84. J. Miao, D. Guo, J. Zhang et al., “Targeted mutagenesis in rice using CRISPR-Cas system,” Cell Research, vol. 23, no. 10, pp. 1233–1236, 2013. View at Publisher · View at Google Scholar · View at Scopus
  85. Q. Shan, Y. Wang, J. Li et al., “Targeted genome modification of crop plants using a CRISPR-Cas system,” Nature Biotechnology, vol. 31, no. 8, pp. 686–688, 2013. View at Publisher · View at Google Scholar
  86. S. K. Upadhyay, J. Kumar, A. Alok, and R. Tuli, “RNA-guided genome editing for target gene mutations in wheat,” G3-Genes Genomes Genetics, vol. 3, no. 12, pp. 2233–2238, 2013. View at Publisher · View at Google Scholar · View at Scopus