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International Journal of Genomics
Volume 2017 (2017), Article ID 6790478, 13 pages
https://doi.org/10.1155/2017/6790478
Research Article

MicroRNA and Putative Target Discoveries in Chrysanthemum Polyploidy Breeding

College of Horticulture, Nanjing Agricultural University, Key Laboratory of Landscaping Agriculture, Ministry of Agriculture, Nanjing 210095, China

Correspondence should be addressed to Nianjun Teng

Received 25 June 2017; Accepted 30 October 2017; Published 13 December 2017

Academic Editor: Marco Gerdol

Copyright © 2017 Fengjiao Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

MicroRNAs (miRNAs), around 22 nucleotides (nt) in length, are a class of endogenous and noncoding RNA molecule that play an essential role in plant development, either by suppressing the transcription of target genes at a transcriptional level or inhibiting translation at a posttranscriptional level. To understand the roles of miRNAs and their target genes in chrysanthemum polyploidy breeding, three sRNA libraries of normal and abnormal embryos after hybridization were performed by RNA-Seq. As a result, a total of 170 miRNAs were identified and there are 41 special miRNAs in cross of paternal chromosome doubling, such as miR169b, miR440, and miR528-5p. miR164c and miR159a were highly expressed in a normal embryo at 18 days after pollination, suggesting the regulatory role at the late stage of embryonic development. miR172c was only detected in the normal embryo at 18 days after pollination, which means that miR172c mainly mediates gene expression in postembryonic development and these genes may promote embryo maturation. Other miRNAs, including miR414, miR2661, and miR5021, may regulate the genes participated in pathways of auxin response and energy metabolism; then they regulate the complex embryonic development together.