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International Journal of Genomics
Volume 2017, Article ID 7305684, 16 pages
Research Article

Comparative Transcriptome Analyses of Resistant and Susceptible Near-Isogenic Wheat Lines following Inoculation with Blumeria graminis f. sp. tritici

1State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, Shandong, China
2Agronomy College, Shandong Agricultural University, Taian, Shandong, China
3College of Life Science, Zaozhuang University, Zaozhuang, Shandong, China

Correspondence should be addressed to Xingfeng Li; nc.ude.uads@fxil

Received 12 December 2016; Revised 8 February 2017; Accepted 6 March 2017; Published 2 May 2017

Academic Editor: Marco Gerdol

Copyright © 2017 Piyi Xing et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Table S1. Quality of the RNA-seq data. 4.16 G, 3.62 G, and 4.06 G of clean data were generated at 16 hpi, and 4.23 G, 3.47 G, and 4.15 G clean data were generated at 96 hpi and more than 90% had read qualities of Q30 (0.1% sequencing error-rate) or higher in Table S1. Table S2. Forty seven co-regulated unigenes with RPKM values of 0 in Chancellor at two timepoints. Among the 1028 co-regulated DEGs between T1 versus T3 and T2 versus T3, 119 DEGs with an RPKM value of 0 in T3 were identified. Meanwhile, 95 DEGs with an RPKM value of 0 in T6 were identified among 2204 overlapping DEGs between T4 versus T6 and T5 versus T6. Following the comparison of these 119 and 95 DEGs, 47 unigenes in Chancellor at both time points were identified with an RPKM value of 0. 47 genes and their putative functions annotated in the non-redundant (nr) and Swissprot databases are listed in Table S2.

  1. Supplementary Material