Application of Genomic In Situ Hybridization in Horticultural Science

Table 1

Genomic in situ hybridization (GISH) application in various horticultural plants.

Plant	Chromosome number	Genomic DNA	Blocking DNA	Working	Reference
Fruit
Cherry	2n = 16	P. avium/P. fruticosa	P. fruticosa/P. avium	Genomic evaluation	[30]
Citrus	2n = 18	C. reticulata (dig labeled)	C. maxima (biotin labeled)	Karyotype analysis of chromosomes	[17]
Diospyros	2n = 30	D. kaki + D. glandulosa	No blocking DNA	Multicolor GISH study of somatic hybrid chromosomes	[18]
Mango	2n = 40	M. caloneura/M. cochinchinensis/M. flava/M. gracilipes/M. griffithii/M. sylvatica/M. indica/M. foetida/M. macrocarpa	No blocking DNA	Phylogenetic division using karyotype tool	[30]
Vegetable
Onion	2n = 40	A. galanthum	A. fistulosum	Gene identification on chromosomal regions	[19]
	2n = 40	A. roylei	A. cepa	Karyotype study and hybridity status in F1 hybrids	[41]
	2n = 40	A. fistulosum	A. cepa	Genomic analysis of advanced interspecfic generations with relative resistance to downy mildew	[62]
Onion & garlic	2n = 40	A. sativum (garlic)	A. cepa (onion)	Chromosome evaluation in onion and garlic somatic hybridization	[35]
Potato	2n = 24	S. verrucosum, S. hougasii, S. andreanum	S. jamesii	Auto and allopolyploid genomic origin in potato species	[29]
Tomato	2n = 24	L. esculentum/S. lycopersicoides/S. sitiens	L. esculentum/S. lycopersicoides/S. sitiens	Genomic discrimination in interspecific and intergeneric hybrids	[40]
Tomato & potato	2n = 24	L. pennellii (tomato)	S. tuberosum (potato)	Genomic analysis of trigenomic hybrids	[38]
Ornamental
Begonia	2n = 28	Tuberous Begonia	B. socotrana	Genomic constituents of begonia hybrids	[39]
Clivia	2n = 22	C. nobilis/C. caulescens/C. gardenii/C. miniata	C. nobilis/C. caulescens/C. gardenii/C. miniata	Hybrid confirmation	[48]
Festulolium (Festuca × Lolium)	2n = 4x = 28	Lolium multiflorum/Festuca pratensis	No blocking DNA	Genomic constitution determination in hybrids	[63]
Lycoris	2n = 14–22	L. aurea/L. radiata	L. aurea/L. radiata	Chromosome complements variation in interspecific hybrids	[51]
Orchids	2n = 38	P. aphrodite/P. sanderiana/P. mannii/P. vialacea/P. ambainensis/P. stuartiana	P. aphrodite/P. sanderiana/P. mannii/P. vialacea/P. ambainensis/P. stuartiana	Genomic composition and species relationship by genomic analysis	[64]
	2n = 26	P. delenatii/P. rothschildianum	P. delenatii/P. rothschildianum	Phylogenetic classification on the basis of chromosome pairing resemblance	[55]
Grass (Poa jemtlandica, Poa flexuosa, Poa alpina)	2n = 38; 2n = 42; 2n = 32	Poa jemtlandica/Poa alpina/Poa flexuosa	Poa alpina/Poa flexuosa	Genome composition and hybrid origin confirmation	[65]
Primula egaliksensis	2n = 40	Primula mistassinica, Primula nutans	Salmon sperm DNA	Genomic composition and evolution of allopolyploid	[66]
Rhododendron	2n = 26	R. aureum, R. brachycarpum, R. catawbiense “Catharine van Tol,” R. yakushimanum	R. aureum, R. catawbiense “Nova Zembla,” R. yakushimanum	Paternity description of interspecific hybrids	[50]
Tulipa	2n = 24	T. fosteriana, T. gesneriana	T. tarda	Genomic recombination in three generations (F1, BC1, and BC2)	[59]
		T. gesneriana and T. fosteriana	Darwin hybrid “Yellow Dover” counter stained with DAPI	Origin investigation by karyotype genomic method	[36]
		T. gesneriana and T. fosteriana	T. sacstatila	Genomic information of interspecific hybrids	[43]
Lilium	2n = 24	Sorbonne and L. regale	Herring sperm	Introgression determination in interpoloid hybrids	[57]
		Oriental/Asiatic/Martagon	Oriental/Asiatic	Backcross progeny analysis	[60]
		L. longiflorum “Snow queen”	Herring sperm	L. rubellum Baker introgression into L. longiflorum Thunb.	[67]
		L. longiflorum	Herring sperm	Genomic evaluation of backcross progenies	[68]