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International Journal of Genomics
Volume 2017 (2017), Article ID 8027626, 10 pages
https://doi.org/10.1155/2017/8027626
Research Article

Transcriptome Analysis of Two Different Developmental Stages of Paeonia lactiflora Seeds

Forestry College, Shenyang Agricultural University, Shenyang, Liaoning 110866, China

Correspondence should be addressed to Xiaomei Sun; moc.621@0827iemoaix

Received 28 November 2016; Revised 13 May 2017; Accepted 5 July 2017; Published 7 August 2017

Academic Editor: Mohamed Salem

Copyright © 2017 Yonglei Ma et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Paeonia lactiflora is a herbaceous flower in the family Paeoniaceae with both hypocotyl and epicotyl dormant seeds. We used high-throughput transcriptome sequencing on two different developmental stages of P. lactiflora seeds to identify seed dormancy and germination-related genes. We performed de novo assembly and annotated a total of 123,577 unigenes, which encoded 24,688 putative proteins with 47 GO categories. A total of 10,714 unigenes were annotated in the KEGG database, and 258 pathways were involved in the annotations. A total of 1795 genes were differentially expressed in the functional enrichment analysis. The key genes for seed germination and dormancy, such as GAI1 and ARF, were confirmed by quantitative reverse transcription-polymerase chain reaction analysis. This is the first report of sequencing the P. lactiflora seed transcriptome. Our results provide fundamental frame work and technical support for further selective breeding and cultivation of Paeonia. Our transcriptomic data also serves as the basis for future genetics and genomics research on Paeonia and its closely related species.