The Role of miRNAs as Biomarkers for Pregnancy Outcomes: A Comprehensive Review

Table 2

Studies reporting an association between alteration of miRNA expression and spontaneous abortion.

Authors and year	Study design	Enrolled population	Samples	Gestational age at sampling (weeks)	Techniques	↑ miRNAs	↓ miRNAs
Wang et al., 2012 [31]	Retrospective	12 cases + 10 controls	Villi	7	Microarray + qRT-PCR	miR-133a
Dong et al., 2014 [34]	Retrospective	20 cases + 15 controls	Villi and decidua	7	Microarray + qRT-PCR	miR-184, miR-187, and miR-125b-2 (villi RPL)—miR-517c, miR-519a-1, miR-522, miR-520h, miR-184 (decidua RPL)	miR-520f, miR-3175, and miR-4672 (villi RPL)
Li and Li., 2016 [11]	Retrospective	20 cases + 20 controls	Decidua	7–10	qRT-PCR	miR-34a, miR-155, miR-141, miR-125a, and miR-125b (RPL)	miR-24 (RPL)
Qin et al., 2016 [13]	Retrospective	27 cases + 28 controls	Maternal plasma	7	miRNA array + qRT-PCR	miR-320b, miR-146b-5p, miR-221-3p, and miR-559 (cases)	miR-101-3p (cases)

qRT-PCR: quantitative real-time polymerase chain reaction; RPL: recurrent pregnancy loss.