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International Journal of Genomics
Volume 2017 (2017), Article ID 9604762, 14 pages
https://doi.org/10.1155/2017/9604762
Research Article

Preparing and Analyzing Expressed Sequence Tags (ESTs) Library for the Mammary Tissue of Local Turkish Kivircik Sheep

1Faculty of Art and Science, Molecular Biology and Genetics, Yıldız Technical University, Istanbul, Turkey
2Department of Biology, Faculty of Art and Science, Ege University, Izmir, Turkey
3Department of Biochemistry, Faculty of Veterinary Medicine, Istanbul University, Istanbul, Turkey
4Department of Surgery, Faculty of Veterinary Medicine, Istanbul University, Istanbul, Turkey

Correspondence should be addressed to Nehir Ozdemir Ozgenturk; moc.oohay@rimedzorihen

Received 18 October 2016; Accepted 7 December 2016; Published 23 January 2017

Academic Editor: Ferenc Olasz

Copyright © 2017 Nehir Ozdemir Ozgenturk et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Kivircik sheep is an important local Turkish sheep according to its meat quality and milk productivity. The aim of this study was to analyze gene expression profiles of both prenatal and postnatal stages for the Kivircik sheep. Therefore, two different cDNA libraries, which were taken from the same Kivircik sheep mammary gland tissue at prenatal and postnatal stages, were constructed. Total 3072 colonies which were randomly selected from the two libraries were sequenced for developing a sheep ESTs collection. We used Phred/Phrap computer programs for analysis of the raw EST and readable EST sequences were assembled with the CAP3 software. Putative functions of all unique sequences and statistical analysis were determined by Geneious software. Total 422 ESTs have over 80% similarity to known sequences of other organisms in NCBI classified by Panther database for the Gene Ontology (GO) category. By comparing gene expression profiles, we observed some putative genes that may be relative to reproductive performance or play important roles in milk synthesis and secretion. A total of 2414 ESTs have been deposited to the NCBI GenBank database (GW996847–GW999260). EST data in this study have provided a new source of information to functional genome studies of sheep.