Genomic context of the Klhl14 S/AS pair in mouse and human. In (a), two genomic segments from mouse and human containing the Klhl14 S/AS pair and flanking genes are displayed. The regions are clearly syntenic, and the presence of an antisense noncoding gene (red circle) is conserved in both species. Pink boxes indicate conserved blocks, and green lines represent their correspondences. In (b) and (c), the slices are zoomed in to show the entire AS genes and the Klhl14 gene portion in overlap for the mouse (b) and human (c) genome, respectively. The constrained elements displayed as grey boxes represent regions of sequence conservation in mammals according to Ensembl. In (b), the regions shared by all the transcripts are indicated by the blue, orange, and green boxes. Fragment A to fragment E represent the isoforms identified in this study with the new ones indicated by the red arrows. The region in the pink rectangle is amplified exclusively with one set of oligos.