Tissue- and Cell Type-Specific Expression of the Long Noncoding RNA Klhl14-AS in Mouse

Figure 4

Cell type-specific expression of Klhl14-AS in adult mouse organs. In situ hybridization was performed on paraffin-embedded sections with a probe recognizing all Klhl14-AS isoforms (for details, see Materials and Methods). (a) The lung, liver, and heart are negative for Klhl14-AS staining. 25x magnifications are shown. (b) The thyroid, kidney, spleen, testis, and ovary are positive for Klhl14-AS staining. For each organ, three different magnifications are shown: 25x, 200x, and 400x. 200x magnifications correspond to the areas boxed in 25x pictures, while 400x magnifications correspond to areas boxed in 200x pictures. RC: renal corpuscle; RP: red pulp; WP: white pulp; L: Leydig cells; ST: seminiferous tubule; OC: oocyte; G: granulosa cells; T: thecal cells. All the images are reduced by 70% compared to the original ones. Data are representative of three independent experiments.

(a)

(b)

(c)