Research Article
Topological Characterization of Human and Mouse m5C Epitranscriptome Revealed by Bisulfite Sequencing
Figure 2
Conservation of m5C in different mRNA regions. (b) Graph showing the status of m5C frequency in different regions of the transcripts. We divided all the detected cytosine sites into 2 groups based on whether it is methylated. The result indicates that cytosine sites with significant methylation levels are consistently enriched at the 5UTRs and near the start codon in all 4 samples. (b) A correlated methylation pattern is observed on 5UTRs between different cell lines/tissues in human and mouse. The conserved cytosine residuals were retrieved with liftOver utility (http://genome.ucsc.edu/cgi-bin/hgLiftOver), and the correlation analysis is performed with Fisher’s exact test. It is important to note that, although we failed to observe a correlated m5C methylation pattern on CDSs and 3UTRs of mRNA, it is possible that such pattern may emerge on strictly matched cell lines/tissues.
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