Research Article

Topological Characterization of Human and Mouse m5C Epitranscriptome Revealed by Bisulfite Sequencing

Figure 2

Conservation of m5C in different mRNA regions. (b) Graph showing the status of m5C frequency in different regions of the transcripts. We divided all the detected cytosine sites into 2 groups based on whether it is methylated. The result indicates that cytosine sites with significant methylation levels are consistently enriched at the 5UTRs and near the start codon in all 4 samples. (b) A correlated methylation pattern is observed on 5UTRs between different cell lines/tissues in human and mouse. The conserved cytosine residuals were retrieved with liftOver utility (, and the correlation analysis is performed with Fisher’s exact test. It is important to note that, although we failed to observe a correlated m5C methylation pattern on CDSs and 3UTRs of mRNA, it is possible that such pattern may emerge on strictly matched cell lines/tissues.