Topological Characterization of Human and Mouse m⁵C Epitranscriptome Revealed by Bisulfite Sequencing

Figure 3

Comparative distributions of mRNA and DNA m⁵C methylation. (a) Bar graph shows the proportion of mRNA m⁵C sites under different combinations of C-centered trinucleotides in mouse and human cells. The dotted line shows the average percentage of methylation under all trinucleotide contexts within the entire transcriptome. We observed that RNA m⁵C occurs under all trinucleotide contexts and is slightly enriched in sequences containing CCG, GCG, GCC, GCU, and GCA. (b) Bar graph showing proportion of DNA m⁵C sites in mouse and human cells. DNA cytosine sites were enriched exclusively in sequences containing CG dinucleotides (ACG, CCG, CCG, and TCG). (c) The coefficient of correlation between RNA methylation and trinucleotide sequences was found to be consistent between samples from the same species (Pearson correlation = 0.96 for human and 0.92 for mouse) and also between human and mouse cells (Pearson correlation = 0.72, 0.75, 0.45, and 0.48).

(a)

(b)

(c)