Research Article

Topological Characterization of Human and Mouse m5C Epitranscriptome Revealed by Bisulfite Sequencing

Figure 4

The methylation ratio of corresponding m5C DNA and mRNA CpG islands shows negative correlation. (a) Negative correlation is observed between DNA and mRNA methylation ratio consistently under all four CG containing trinucleotides (ACG, TCG, CCG, and GCG) in both human and mouse, that is, if a specific CG dinucleotide in DNA is methylated, the corresponding dinucleotide in mRNA is significantly less likely to be methylated. The top 4 nucleotide contexts under which the strongest correlation between DNA and RNA methylation level exists. (b) Comparative distributions of m5C methylated CG sites in DNA and RNA show an enrichment of sites with a high methylation ratio in mRNA 5UTR as opposed to an enrichment of low-methylation-ratio sites in DNA 5UTR. The pattern is consistent in both the human MCF10A cell line and mouse embryo stem cells.