Topological Characterization of Human and Mouse m⁵C Epitranscriptome Revealed by Bisulfite Sequencing

Figure 6

m⁵C is enriched on mRNAs transcribed from mitochondrial DNA. (a) Bar graph depicting m⁵C mRNA methylation sites on different chromosomes. RNAs transcribed from mitochondrial DNA (M) showed drastically increased frequency of m⁵C sites (enrichment odds ratio of 818.42949, 634.72723, 1028.52065, and 67.28553). (b) Bar graph showing the number of methylated cytosine reads stacked with unmodified cytosine reads generated from 6 major classes of mitochondrial genes. The RNA transcripts of all the major genes located on a mitochondrial chromosome were significantly methylated.

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