Research Article

Topological Characterization of Human and Mouse m5C Epitranscriptome Revealed by Bisulfite Sequencing

Figure 6

m5C is enriched on mRNAs transcribed from mitochondrial DNA. (a) Bar graph depicting m5C mRNA methylation sites on different chromosomes. RNAs transcribed from mitochondrial DNA (M) showed drastically increased frequency of m5C sites (enrichment odds ratio of 818.42949, 634.72723, 1028.52065, and 67.28553). (b) Bar graph showing the number of methylated cytosine reads stacked with unmodified cytosine reads generated from 6 major classes of mitochondrial genes. The RNA transcripts of all the major genes located on a mitochondrial chromosome were significantly methylated.