Research Article
The β-Lactamase Gene Profile and a Plasmid-Carrying Multiple Heavy Metal Resistance Genes of Enterobacter cloacae
Table 1
Bacteria and plasmids used in this study.
| | Strain | Relevant characteristic(s) | Reference or source |
| | BL21 | E. coli BL21 was used as a host for the cloned blaMIR gene | [14] | | ATCC25922 | E. coli ATCC25922 is FDA clinical isolate | [14] | | CG34 | blaMIR-producing isolate of E. cloacae CG34 | This study | | CG76 | blaMIR-producing isolate of E. cloacae CG76 | This study | | CG85 | blaMIR-producing isolate of E. cloacae CG85 | This study | | Y546 | blaMIR-producing isolate of E. cloacae Y546 | This study | | Y482 | blaMIR-producing isolate of E. cloacae Y482 | This study | | Y490 | blaMIR-producing isolate of E. cloacae Y490 | This study | | pET28a/BL21 | BL21 carrying expression vector pET28a, kmr | This study | | pET28a-blaMIR-CG34/BL21 | BL21 carrying recombinant plasmid pET28a-blaMIR-CG34 | This study | | pET28a-blaMIR-Y490/BL21 | BL21 carrying recombinant plasmid pET28a-blaMIR-Y490 | This study | | pET28a-blaMIR-CG76/BL21 | BL21 carrying recombinant plasmid pET28a-blaMIR-CG76 | This study | | pET28a-blaMIR-Y546/BL21 | BL21 carrying recombinant plasmid pET28a-blaMIR-Y546 | This study | | pET28a-blaMIR-CG85/BL21 | BL21 carrying recombinant plasmid pET28a-blaMIR-CG85 | This study | | pET28a-blaMIR-Y482/BL21 | BL21 carrying recombinant plasmid pET28a-blaMIR-Y482 | This study | | pET28a-blaCTX-M-9a-Y546/BL21 | BL21 carrying recombinant plasmid pET28a-blaCTX-M-9a-Y546 | This study | | pET28a-blaSHV-12-Y546/BL21 | BL21 carrying recombinant plasmid pET28a-blaSHV-12-Y546 | This study |
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km: kanamycin; r: resistant.
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