The β-Lactamase Gene Profile and a Plasmid-Carrying Multiple Heavy Metal Resistance Genes of Enterobacter cloacae

Table 2

Primers used in the study for the detection of β-lactamase-encoding genes.

Primer	Target(s)	Sequence (5-3)	Annealing temperature (°C)	Amplicon size (bp)	Reference
veb-sf	blaVEB	GATTGCTTTAGCCGTTTTGTC	50	452	[15]
veb-sr		ATCGGTTACTTCCTGTTGTTGTTTC
z-sf	blaZ	ACAGTTCACATGCCAAAGAGT	50	479	[16]
z-sr		CTTACCGAAAGCAGCAGGTG
mir-sf	blaMIR	GCCGCACCGATGTCCGAAAAA	50	545	[18]
mir-sr		GGTTTAAAGACCCGCGTCGTCATGG
azecl-29-sf	blaAZECL-29	GTCTTTACGCTAACACCAGCATCGG	50	381	[17]
azecl-29-sr		TCAGCATTTCCCAGCCCAATC
veb-ff	blaVEB	CGGGATCCATGAAAATCGTAAAAAGGATATTAT	50	918	This study
veb-fr		CCCAAGCTTTATTTATTCAAATAGTAATTCCACG
z-ff	blaZ	CGGGATCCATGAAAAAGTTAATATTTTTAATTG	50	864	This study
z-fr		CCCAAGCTTTAAAATTCCTTCATTACACTCTTG
mir-ff	blaMIR	CGGGATCCATGATGACAAAATCCCTAAGCTGTG	66	1164	This study
mir-fr		CCCAAGCTTTACTGCAGCGCGTCGAGGATACGG
azecl-29-ff	blaAZECL-29	CGGAATTCATGATGAAAAAAAACCTAAGCTGTG	60	1164	This study
azecl-29-fr		CCCAAGCTTTACTGCAGCGCGTCGAGGATACG
ctx-m-9a-ff	blaCTX-M-9a	CGGGATCCATGGTGACAAAGAGAGTGCAACGGA	60	876	This study
ctx-m-9a-fr		CCAAGCTTTTACAGCCCTTCGGCGATGATTCTC
shv-12-ff	blaSHV-12	CGGGATCCGTGGTTATGCGTTATATTCGCCTGT	60	867	This study
shv-12-fr		CCAAGCTTTTAGCGTTGCCAGTGCTCGATCAGC

Underlined sequences denote restriction endonuclease sites. sf: forward screening primer; sr: reverse screening primer; ff: forward full-length primer; fr: reverse full-length primer.