Overexpression of a New Zinc Finger Protein Transcription Factor OsCTZFP8 Improves Cold Tolerance in Rice

Figure 1

Analyses of OsCTZFP8 protein architecture and its relationship to other zinc finger protein in plants. (a) Schematic representation of the 225 amino acids of OsCTZFP8. Two exons, one intron, NLS, L-box, and C₂H₂ zinc finger domain are indicated. (b) Amino acid sequence alignment of rice OsCTZFP8 with zinc finger proteins from rice Osl_28710 (Oryza sativa L., gi|34015350), maize ZmZFP1 (Zea mays, gi|242032883), Arabidopsis AtZFP1 (Arabidopsis thaliana, gi|15240742), soybean GsZAT10 (Glycine soja, gi|734330588), and winter oilseed rape ZFP1 (Brassica napus, gi|685279228) was performed using a NCBI constraint-based multiple alignment tool and visualized with GeneDoc version 2.7. One zinc finger motif, putative NLS, and L-Box are indicated. Identical and conserved amino acids are displayed in black and grey backgrounds, respectively. (c) The phylogenetic tree of OsCTZFP8 homologs in plants. Phylogenetic analysis was conducted by MEGA version 4.0 using fourteen OsCTZFP8 homologs selected from multiple sequence alignment. A neighbor-joining tree was built using the bootstrap method with 1000 replicates. The numbers at each node represent the bootstrap percentage. In addition to the members cited in (b), the following protein sequences were incorporated into the analysis: rice Os07g0209600 (Oryza sativa japonica, gi|937925668), rice OsZF1 (Oryza sativa japonica, gi|34393438), rice C₂H₂ transcription factor (Oryza sativa japonica, gi|323388891), rice OsJ_12692 (Oryza sativa indica, gi|125588016), maize putative zinc-finger protein1 (Zea mays, gi|195640880), Sorghum SbZFP1 (Sorghum bicolor, gi|670405684), Arabidopsis C₂H₂ and C₂HC zinc fingers superfamily protein (Arabidopsis thaliana C₂H₂ and C₂HC zinc fingers superfamily protein, gi|15229643), and Brassica BrAZF1 (Brassica rapa, gi|923538325).

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