Mutations in Homocysteine Metabolism Genes Increase Keratin N-Homocysteinylation and Damage in Mice

Table 3

Hcy-keratin and S-Hcy-keratin levels and the solubility of hair Hcy-keratin in HHcy and control wild-type mice.

Genotype ()	Hcy-keratin (pmol/mg hair)	S-Hcy-keratin (pmol/mg hair)	Hcy-keratin/(Hcy-keratin + S-Hcy-keratin)	SDS-soluble Hcy-keratin fraction
Cbs^−/− (4)	1014 ± 86	1937 ± 336	0.35 ± 0.03	0.07 ± 0.03
Cse^−/− (5)	546 ± 46	682 ± 144	0.55 ± 0.04	0.14 ± 0.01
Mthfr^−/− (7)	302 ± 71^#	248 ± 121^#	0.57 ± 0.17	0.19 ± 0.03^#
Wild type + Met (6)	97 ± 11	303 ± 58	0.24 ± 0.02	0.31 ± 0.07
Wild type (28)	87 ± 10	127 ± 10	0.41 ± 0.21	0.36 ± 0.09

^∗,#Significantly different from wild type; ,