Expression Analysis of Nitrogen Metabolism-Related Genes Reveals Differences in Adaptation to Low-Nitrogen Stress between Two Different Barley Cultivars at Seedling Stage

Table 1

Primers for qRT-PCR.

Gene name	Accession number	Prime sequences (5 to 3)		Amplicon (bp)	Origin
HvNRT2.2	U34290.1	Forward	TCCTTCTTCACCTGCTTCGT	80	This study
		Reverse	TTGGCGAGGTTTAGGTTGTC
HvNRT2.3	AF091115.1	Forward	ATGGCGTATTGCCTACTTCG	90
		Reverse	TTCCCATCAGGGAGATCTTG
HvNRT3.1	AY253448.1	Forward	GAACGTGAAGGTGAGCCTCT	96
		Reverse	TGGCAGGTCTTGTCCTTCTT
HvNRT3.3	AY253450.1	Forward	AAGGACGCCGACTACAAGAA	131
		Reverse	TGCTGGGTGATCTTGAACTG
HvNIA2	X57845.1	Forward	TGGCAAGAAGATCACACGAG	120
		Reverse	CAGAAGCACCAGCACCAGTA
HvNiR1	S78730.1	Forward	CTCACCGGGGTGTACAAGAA	114
		Reverse	CTCCTCGTCCTCCTCCCTCT
HvGS1_1	X69087.1	Forward	GTTCAGGGAGGGAAACAACA	112
		Reverse	ATCGGGGTTGCTAAGGATCT
HvGS2	X53580.1	Forward	ATAGCCGCATATGGTGAAGG	106
		Reverse	GAATAGAGCAGCCACGGTTC
HvGLU2	S58774.1	Forward	ACCAATGAGGTTGCTTGGAC	85
		Reverse	TATTGTGGCTTCCCTTGACC
HvASN1	AF307145.1	Forward	AAGGAGGGAGGCTTCAAGAG	146
		Reverse	AGAACACCGAATGGAACGTC
HvActin	AY145451.1	Forward	TGAGGCGCAGTCCAAGAGA	81	Chen et al. [26]
		Reverse	TCCATGTCATCCCAGTTGCTTA
HvGAPDH	X60343.1	Forward	ACAGTTCACGGCCATTGGA	102
		Reverse	AGGGTTCCTGACGCCAAAG