Discovery of Novel Caeridins from the Skin Secretion of the Australian White’s Tree Frog, Litoria caerulea

Figure 10

Membrane permeabilisation of human microvascular endothelial cell line, HMEC-1, reflected by the level of LDH released into the medium, after being treated by (a) Caeridin-1, (b) S5-Caeridin-1, (c) Caeridin-a1, (d) positive peptide melittin, and (e) negative peptide Bradykinin. The level of LDH release was calculated in comparison with the maximum LDH release control group (lysis buffer-treated)). represents a significant change in the level of LDH release, compared with the maximum LDH release control group (lysis buffer-treated), using one-way analysis of variance followed by Dunnett’s multiple comparisons tests. The error bar represents SEM of three independent experiment determinations and each experiment with triplicates.

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