Transcriptome Analysis Reveals Dynamic Fat Accumulation in the Walnut Kernel

Table 1

qRT-PCR validation primers.

Gene	Primer sequence (5 to 3)	Tm (°C)
β-Actin	CTCTTCCAGCCATCCATGATCG	57.63
	CCACTGAGGACAATATTGCCAT	55.16
LACS7	CAGGAGTTGAGGTTGTGACATATTC	56.05
	AGAGCAGCCATATCATCCATTAGTT	56.28
LACS1	CAGGTTAGGTGGTCGGATTAGACT	57.86
	TGATGGCTCCTCAAGTGGATTGT	58.43
ACC1	GAGAATTGCTGAAGAGTCGCTGAT	57.73
	TGTTGGCTCCACCTTGCTTAGA	58.56
FAD7	ACTTCAACTCTGTGCTGGTCTCT	58.07
	CCTCCTCGTAGATAACTCCATTCCT	57.29
LACS9	CGACTTACCACTTCCAGCCGATA	58.66
	ACTTCCAACAGCAGCAATCACATT	58.22
ACX5	TGATGAGGGTTTCACAGGTTACAAG	57.32
	AAGCATAAGCAGAAGCCAGCAA	57.86
PKT3	GCAGGATGGCGGCATTCTATG	58.74
	AGAGGCAGTAGCAGCAGCAG	59.29
PAS2	CGTCTTCTTCGGATGGCTTCA	57.33
	CAGTGTTGCTGAGATCGGAGAT	56.92