Calibrating Transcriptional Activity Using Constitutive Synthetic Promoters in Mutants for Global Regulators in Escherichia coli

Figure 1

Construction and validation of the reporter systems. (a) Synthetic promoters were cloned into the plasmid pMR1, which contains a short-lived GFPlva variant, and pGLR2, a broad host range vector containing a GFP-luxCDABE reporter system. (b) Maximal promoter activity of the four promoters in pMR1 vector. (c) Maximal promoter activity analyzed by monitoring lux expression using pGLR2 constructions. (d) GFP expression profile along the growth curve from reporters cloned in pMR1 vector. (e) lux expression profile along the growth curve from reporters cloned in pGLR2 vector. The solid lines represent the average values calculated using data from three independent experiments while dashed lines represent standard deviation from the samples.

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