Calibrating Transcriptional Activity Using Constitutive Synthetic Promoters in Mutants for Global Regulators in Escherichia coli

Figure 3

Quantification of promoter activities in different E. coli strains. (a) Representation of the sequences at −10 or −35 for the four constitutive promoters analyzed, using bold letters for bases conserved related to Pj100 reference. (b) Promoter activity of E. coli strains harboring a Plac::GFPlva fusion in minimal medium with 1% glycerol (left) or 1%glycerol plus 0.4% glucose (right) as carbon source. (c) Promoter activity of E. coli strains harboring different promoter fusions (Pj100, Pj106, Pj114, and Pj113) in minimal media with 1% glycerol or 1%glycerol plus 0.4% glucose (labeled as glu) as carbon source. Solid lines represent the average values calculated using data from three independent experiments for wild type (black), Δihf (red), and Δfis (green) strains, while dashed lines represent the upper and lower limits of standard deviations.

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