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Strains, plasmids, and primers | Description | Reference |
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Strains |
E. coli DH5α | F− endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG purB20 φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK−mK+), λ−. | [12] |
E. coli DH10B | mcrA Δmrr-hsdRMS-mcrBC) φ 80lacZΔM15 ΔlacX74 recA1 araD139 Δ (ara-leu)7697 galU galK rpsL endA1 nupG Δdcm. | [12] |
E. coli BW25113 | lacI+rrnBT14 ΔlacZWJ16 hsdR514 ΔaraBADAH33 ΔrhaBADLD78 rph-1 | [12] |
Δ(araB–D)567 Δ(rhaD–B)568 ΔlacZ4787(::rrnB-3) hsdR514 rph-1. |
E. coli JW1702 | E. coli BW25113 Δihf mutant | [12] |
E. coli JW3229 | E. coli BW25113 Δfis mutant | [12] |
Plasmids |
pMR1 | CmR, ori p15a. GFPlva promoter probe vector | [14] |
pMR1-Pj113 | pMR1 with Pj113 cloned as EcoRI/BamHI fragment | This work |
pMR1-Pj114 | pMR1 with Pj114 cloned as EcoRI/BamHI fragment | This work |
pMR1-Pj106 | pMR1 with Pj106 cloned as EcoRI/BamHI fragment | This work |
pMR1-Pj100 | pMR1 with Pj100 cloned as EcoRI/BamHI fragment | This work |
pMR1-Plac | pMR1 with Plac promoter cloned as EcoRI/BamHI fragment | [28] |
pGLR2 | KmR, oriT, ori RK2. SEVA-based vector with dual GFP-lux reporter | [15] |
pGLR2-Pj113 | pGLR2 with Pj113 cloned as EcoRI/BamHI fragment | This work |
pGLR2-Pj114 | pGLR2 with Pj114 cloned as EcoRI/BamHI fragment | This work |
pGLR2-Pj106 | pGLR2 with Pj106 cloned as EcoRI/BamHI fragment | This work |
pGLR2-Pj100 | pGLR2 with Pj100 cloned as EcoRI/BamHI fragment | This work |
pMR1-xylS-Pm | pMR1 with PxylS, xylS and Pm cloned as EcoRI/BamHI fragment | This work |
Primers |
Pj100-FW | GAATTCTTGACGGCTAGCTCAGTCCTAGG | This work |
Pj100-RV | TACAGTGCTAGCAAGTGGATCCTTGCGATC | This work |
Pj106-FW | GAATTCTTTACGGCTAGCTCAGTCCTAGGTA | This work |
Pj106-RV | TAGTGCTAGCAAGTGGATCCTTGCGATC | This work |
Pj114-FW | GAATTCTTTATGGCTAGCTCAGTCCTAGGT | This work |
Pj114-RV | ACAATGCTAGCAAGTGGATCCTTGCGATC | This work |
Pj113-FW | GAATTCCTGATGGCTAGCTCAGTCCTAGGG | This work |
Pj113-RV | ATTATGCTAGCAAGTGGATCCTTGCGATC | This work |
5_xylS_EcoRI | GAATTCTCAAGCCACTTCCTTTTTGCATTG | This work |
3_Pm_BamHI | GGATCCATTATTGTTTCTGTTGCATAAAGCC | This work |
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