−254C>G SNP in the TRPC6 Gene Promoter Influences Its Expression via Interaction with the NF-κB Subunit RELA in Steroid-Resistant Nephrotic Syndrome Children

Figure 2

Differential analysis of the binding of the transcription factor NF-κB to the TRPC6 promoter region in podocytes and HEK 293T cells using ChIP-PCR. Five primers were designed to amplify different DNA fragments residing in the desired promoter region of the TRPC6 gene (shown as P1-5), and the DNA-protein complex (with −254C or G allele) was precipitated using negative control IgG or antibodies against NF-κB. Both in podocytes and HEK 293T cells, NF-κB was bound to the TRPC6 promoter with −254G (P5), while no binding was found in the normal sequence. TSS: transcription start site.